Team:BostonU/Data

From 2012.igem.org

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<h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose <i>mmoR</i> and a &sigma;<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a &sigma;<sup>54</sup>-dependent transcriptional activator.  
<h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose <i>mmoR</i> and a &sigma;<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a &sigma;<sup>54</sup>-dependent transcriptional activator.  
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It is unclear if the &sigma;<sup>54</sup> promoter will function in <i>E. coli</i> in the absence of <i>mmoR</i>. nBLAST results comparing the <i>mmoR</i> to the <i>E. coli</i> genomes available yielded very short hits, with the longest match under 200bp out of a possible 2070bp length of <i>mmoR</i>. However, the pBLAST hits showed stronger hits with other &sigma;<sup>54</sup>-dependent transcriptional activators found in <i>E. coli</i>.
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It is unclear if the &sigma;<sup>54</sup> promoter will function in <i>E. coli</i> in the absence of <i>mmoR</i>. nBLAST results comparing the <i>mmoR</i> to the <i>E. coli</i> genomes available yielded very short hits, with the top hit showing matches for less than 200bp out of the possible 2070bp length of <i>mmoR</i>. However, the pBLAST hits showed stronger hits with other &sigma;<sup>54</sup>-dependent transcriptional activators found in <i>E. coli</i>. The top nBLAST and pBLAST hits are shown below. <br><br>
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To determine if the &sigma;<sup>54</sup> promoter will function without <i>mmoR</i> present in <i>E. coli</i>, we placed the promoter in front of GFP, RFP, or YFP.
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Revision as of 19:57, 2 October 2012

BostonU iGEM Team: Welcome


Data Collected


    Sequencing Data for Converted MoClo Parts


    Characterizing BioBrick Based Devices









    Characterizing MoClo and BioBrick Based Devices



    Characterizing New BioBrick Parts
      A New Repressible System: Copper and mmoR

        We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose mmoR and a σ54 promoter associated with it from a bacterium called Methylosinus trichosporium OB3b. mmoR is a σ54-dependent transcriptional activator.

        It is unclear if the σ54 promoter will function in E. coli in the absence of mmoR. nBLAST results comparing the mmoR to the E. coli genomes available yielded very short hits, with the top hit showing matches for less than 200bp out of the possible 2070bp length of mmoR. However, the pBLAST hits showed stronger hits with other σ54-dependent transcriptional activators found in E. coli. The top nBLAST and pBLAST hits are shown below.



        To determine if the σ54 promoter will function without mmoR present in E. coli, we placed the promoter in front of GFP, RFP, or YFP.



        Scanlan et. al, 2009

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