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Team:BostonU/Data
From 2012.igem.org
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<h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose <i>mmoR</i> and a σ<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a σ<sup>54</sup>-dependent transcriptional activator. | <h7>We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose <i>mmoR</i> and a σ<sup>54</sup> promoter associated with it from a bacterium called <i>Methylosinus trichosporium</i> OB3b. <i>mmoR</i> is a σ<sup>54</sup>-dependent transcriptional activator. | ||
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| - | It is unclear if the σ<sup>54</sup> promoter will function in <i>E. coli</i> in the absence of <i>mmoR</i>. | + | It is unclear if the σ<sup>54</sup> promoter will function in <i>E. coli</i> in the absence of <i>mmoR</i>. nBLAST results comparing the <i>mmoR</i> to the <i>E. coli</i> genomes available yielded very short hits, with the longest match under 200bp out of a possible 2070bp length of <i>mmoR</i>. However, the pBLAST hits showed stronger hits with other σ<sup>54</sup>-dependent transcriptional activators found in <i>E. coli</i>. |
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<center> | <center> | ||
Revision as of 19:51, 2 October 2012
Data Collected
It is unclear if the σ54 promoter will function in E. coli in the absence of mmoR. nBLAST results comparing the mmoR to the E. coli genomes available yielded very short hits, with the longest match under 200bp out of a possible 2070bp length of mmoR. However, the pBLAST hits showed stronger hits with other σ54-dependent transcriptional activators found in E. coli.
