Team:Washington/Protocols/PAGE gel electrophoresis

From 2012.igem.org

(Difference between revisions)
(Created page with "=SDS PAGE Gel Electrophoresis= == General Procedure == #Place gel cartridge in gel box with loading buffer #Load samples #Run the gel #Place gel in DI water for one hour #Remov...")
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#Place gel in DI water for one hour
#Place gel in DI water for one hour
#Remove water, add staining agent (GelCode Blue), leave on rocker overnight
#Remove water, add staining agent (GelCode Blue), leave on rocker overnight
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#Destain gel with DI water
+
#Remove GelCode Blue, add DI water. Refresh DI water every hour until gel bands are visible.

Revision as of 00:50, 2 October 2012

SDS PAGE Gel Electrophoresis

General Procedure

  1. Place gel cartridge in gel box with loading buffer
  2. Load samples
  3. Run the gel
  4. Place gel in DI water for one hour
  5. Remove water, add staining agent (GelCode Blue), leave on rocker overnight
  6. Remove GelCode Blue, add DI water. Refresh DI water every hour until gel bands are visible.
Retrieved from "http://2012.igem.org/Team:Washington/Protocols/PAGE_gel_electrophoresis"