Team:Washington/Protocols

From 2012.igem.org

(Difference between revisions)
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[https://2012.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
[https://2012.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
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[https://2012.igem.org/Team:Washington/Protocols/PAGE_gel_electrophoresis SDS PAGE gel Electrophoresis]
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[https://2012.igem.org/Team:Washington/Protocols/PAGE_gel_electrophoresis General SDS PAGE gel Electrophoresis]
[https://2012.igem.org/Team:Washington/Protocols/PCR  General PCR Protocol]
[https://2012.igem.org/Team:Washington/Protocols/PCR  General PCR Protocol]

Revision as of 00:42, 2 October 2012


General Protocols

General Agarose Gel Electrophoresis

General SDS PAGE gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Gibson Cloning/Assembly

Isolation of Plasmid DNA (miniprep)

Preparation of Overnight Cultures

PCR Purification

Gel Purification

Plastic Degradation

osmY Assay

Flu Binders

Kunkel Mutagenesis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]

Yeast Surface Display

Yeast Transformation

Optogenetics

Light App Experiments

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