Team:Carnegie Mellon/Bio-Properties

From 2012.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 106: Line 106:
<!--Table of Contents -->
<!--Table of Contents -->
 +
<!-- Remove for testing
<div id="toc-holder" class="toc-holder">
<div id="toc-holder" class="toc-holder">
<a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a>
<a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a>
Line 118: Line 119:
</ul>
</ul>
-
</div><!-- .toc-holder -->
+
</div>
 +
-->
 +
<!-- .toc-holder -->
   <div class = "main_content">
   <div class = "main_content">
 +
<h1>Delete this page: Properties of Promoters</h1>
 +
<p>
 +
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
 +
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
 +
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br>
 +
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
 +
</p>
 +
 +
Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
 +
 +
<table border="1">
 +
 +
<tr>
 +
<th>Promoter</th>
 +
<td> Mutant I </td>
 +
<td> Mutant II </td>
 +
<td> Mutant III </td>
 +
</tr>
 +
 +
<tr>
 +
<th>Transcription Strength</th>
 +
<td> 97% </td>
 +
<td> 72% </td>
 +
<td> 127% </td>
 +
 +
</tr>
 +
 +
<tr>
 +
<th>Translational Efficiency</th>
 +
<td> 6%</td>
 +
<td> 6%</td>
 +
<td> 94%</td>
 +
 +
</tr>
 +
 +
<tr>
 +
<th>RNA degradation constant (assumed)</th>
 +
<td> 100% </td>
 +
<td> 100% </td>
 +
<td> 100% </td>
 +
 +
</tr>
 +
 +
<tr>
 +
<th>Protein degradation constant (fit)</th>
 +
<td> 4% </td>
 +
<td> 6% </td>
 +
<td> 60% </td>
 +
 +
</tr>
 +
</table>
</html>
</html>
{{:Team:Carnegie_Mellon/Templates/Footer}}
{{:Team:Carnegie_Mellon/Templates/Footer}}

Latest revision as of 21:16, 1 October 2012

Image:CMU_image6.jpeg




Delete this page: Properties of Promoters

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter.
Promoter Mutant I Mutant II Mutant III
Transcription Strength 97% 72% 127%
Translational Efficiency 6% 6% 94%
RNA degradation constant (assumed) 100% 100% 100%
Protein degradation constant (fit) 4% 6% 60%

Image:TartanFooter.jpeg

Retrieved from "http://2012.igem.org/Team:Carnegie_Mellon/Bio-Properties"