Team:Carnegie Mellon/Bio-Properties
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+ | <a href="https://2012.igem.org/Team:Carnegie_Mellon/Mod-Overview">Modeling</a> | ||
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+ | <h1>Delete this page: Properties of Promoters</h1> | ||
+ | <p> | ||
+ | BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br> | ||
+ | Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br> | ||
+ | Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br> | ||
+ | Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br> | ||
+ | </p> | ||
+ | |||
+ | Using the model we developed in MATLAB, we calculated the following properties with respect to the wild-type promoter. | ||
+ | |||
+ | <table border="1"> | ||
+ | |||
+ | <tr> | ||
+ | <th>Promoter</th> | ||
+ | <td> Mutant I </td> | ||
+ | <td> Mutant II </td> | ||
+ | <td> Mutant III </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th>Transcription Strength</th> | ||
+ | <td> 97% </td> | ||
+ | <td> 72% </td> | ||
+ | <td> 127% </td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th>Translational Efficiency</th> | ||
+ | <td> 6%</td> | ||
+ | <td> 6%</td> | ||
+ | <td> 94%</td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th>RNA degradation constant (assumed)</th> | ||
+ | <td> 100% </td> | ||
+ | <td> 100% </td> | ||
+ | <td> 100% </td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th>Protein degradation constant (fit)</th> | ||
+ | <td> 4% </td> | ||
+ | <td> 6% </td> | ||
+ | <td> 60% </td> | ||
+ | |||
+ | </tr> | ||
+ | </table> | ||
</html> | </html> | ||
{{:Team:Carnegie_Mellon/Templates/Footer}} | {{:Team:Carnegie_Mellon/Templates/Footer}} |
Latest revision as of 21:16, 1 October 2012
Delete this page: Properties of Promoters
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA
Promoter | Mutant I | Mutant II | Mutant III |
---|---|---|---|
Transcription Strength | 97% | 72% | 127% |
Translational Efficiency | 6% | 6% | 94% |
RNA degradation constant (assumed) | 100% | 100% | 100% |
Protein degradation constant (fit) | 4% | 6% | 60% |