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Team:Carnegie Mellon/Bio-Submitted
From 2012.igem.org
(Difference between revisions)
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<h1 class="section1"> Submitted Parts </h1> | <h1 class="section1"> Submitted Parts </h1> | ||
<p> | <p> | ||
| + | We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs. | ||
| + | |||
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br> | BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br> | ||
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br> | Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br> | ||
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Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br> | Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br> | ||
</p><p class="promoter"> | </p><p class="promoter"> | ||
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| - | Expected promoter strength of | + | <h3 class="pre-experiment">Predicted strength of the hybrid T7Lac promoters</h3> |
| + | |||
| + | Expected promoter strength of the mutants (%relative strength as compared to the wild type T7Lac promoter BBa_K613007):<br> | ||
Mutant I: <100% <br> | Mutant I: <100% <br> | ||
Mutant II: ~100% <br> | Mutant II: ~100% <br> | ||
Revision as of 21:06, 1 October 2012
Submitted Parts
We have submitted three T7Lac promoter parts to the registry. The followings show the sequences of these constructs.
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA
Predicted strength of the hybrid T7Lac promoters
Expected promoter strength of the mutants (%relative strength as compared to the wild type T7Lac promoter BBa_K613007):Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%
Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average
