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Team:Caltech/Notebook/Proteorhodopsin
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Revision as of 18:49, 1 October 2012
Proteorhodopsin Notebook
6/22/12
Julia - transformed K572005 (proteorhodopsin gene)
Daisy - ordered primers for Polymerase Cycling Assembly (PCA) reaction to create a proteorhodopsin optimized for E. coli
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6/25/12
Chenxi - Transformed pKD46 into wild type MG1655 E.coli strain using electroporation; designed primers to PCR pKD4 Kan resistance gene for lambda red knockout of nuo and ndh genes (NADH dehydrogenases)
Julia - sequenced K572005
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6/26/12
Chenxi - PCR pKD4 kan for nuo and ndh; ran gel
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6/27/12
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6/28/12
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6/29/12
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6/30/12
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7/1/12
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7/2/12
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7/3/12
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