Team:MIT/Results

From 2012.igem.org

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{{MIT-header2}}
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DEPRECATED.  DO NOT EDIT.
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{{MIT-style-team}}
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<body>
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<div id="col_nav">
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    <div id="accordion">
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<h3><a href="#">Overview</a></h3>
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<div class="col_list">
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<ul>
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    <li id="aa1">Placeholder</li>
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    </div><!-- end overview -->
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<h3><a href="#">Biobricks</a></h3>
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<h3><a href="#">Foundational</a></h3>
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<ul>
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    <li id="b1">In Vitro </li>
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    <li id="b2">Nucleic Acid Delivery</li>
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            <li id="b3">In Vivo Strand Displacement </li>
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</ul>
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    </div><!-- end foundational -->
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<h3><a href="#">Sensing</a></h3>
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          <ul>
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            <li id="iv1">Modeling</li>
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            <li id="iv2">In Vitro Sensing</li>
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          </ul>
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        </div><!-- end sensing-->
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<h3><a href="#">Processing</a></h3>
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          <ul>
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            <li id="m1"> Modeling </li>
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            <li id="m2"> In Vitro Not Gate </li>
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            <li id="m3"> Hammerhead Ribozymes </li>
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        </div><!-- end processing-->
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<h3><a href="#">Actuation</a></h3>
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          <ul>
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            <li id="m4"> Modeling </li>
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            <li id="m5"> In Vitro  </li>
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            <li id="m6"> In Vivo </li>
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<div class="bio" id="b1bio">
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<b> [RNA Strand Displacement In Vitro] </b>
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<br>
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<br>
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<br> Our team first decided to repeat the results of Winfree / Qian's 2011 paper on strand displacement, but using 2'-O-Methyl RNA tagged with fluorophores and quenchers.
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<br>
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<b> Figure A </b>
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<img src="https://static.igem.org/mediawiki/2012/e/ea/Foundation1MIT.png"/>
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</div>
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<b> Nucleic Acid Delivery </b>
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<br>
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<br>
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<br>
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<b> Delivery of Plasmid DNA to Mammalian Cells </b>
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<br>
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<br> Constitutive Expression of fluorescent markers etc
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<br>
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<b> Delivery of 2'-O-Me RNA to Mammalian Cells </b>
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<br>
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<br> Movie of HEK293 cell being transfected with T*-S6*-ROX, as well as static images
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<br> 15,20,25,30 pmol ratio data from FACS
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<br>
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<br>
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<b> Inducible Control of Protein Expression </b>
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<br> DOX curve
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<b> Delivery of Plasmid DNA which transcribes short RNA Inputs </b>
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<br>
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<br> FF1 Knockdown Data
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</div>
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<div class="bio" id="b3bio">
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<b>In Vivo RNA Strand Displacement </b>
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<br>
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<br>
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<b> Strategy 1: Lipofectamine 2000 Transfection of RNA version of Reporter from Winfree/QIan 2011 Paper</b>
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<br>
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<br>
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<br> Images will go here from April 24th experiment  - display red fluorescence in all wells, including those that only got reporter or the wrong input - also see red vesicles indicating reporter comes apart inside the vesicles
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<br>
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<br>
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<br> Strategy 2: Switch Transfection reagent to RNAiMAX </b>
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<br>
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<br> RNAiMAX is supposed to be a better transfection reagent for double stranded RNA
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<br>
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<br> Images will go here from experiment from June 13th onward where we do not see red vesicles, however we still see whole cell red fluorescence
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<br>
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<br>
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<b> Strategy 3: Tag RNA strand with an Alexa Fluorophore to act as a transfection marker </b>
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<br>
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<b> Strategy 4: Create DNA plasmids driving transcription of RNA inputs, while transfecting RNA Reporter </b>
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<br>
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<br>
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<b> Strategy 4: Nucleofect RNA reporter, RNA inputs </b>
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<br>
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<b> [Strategy 5]: Redesign RNA Reporter </b>
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</div>
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<div id='next' style="float:right; margin-top: 20px;"> </div>
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</body>
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</html>
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Latest revision as of 16:41, 1 October 2012

DEPRECATED. DO NOT EDIT.

Retrieved from "http://2012.igem.org/Team:MIT/Results"