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Team:Cornell/Notebook
From 2012.igem.org
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*[[Team:Cornell/Notebook/Nah operon|nah operon]] -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter. | *[[Team:Cornell/Notebook/Nah operon|nah operon]] -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter. | ||
*[[Team:Cornell/Notebook/Salicylate reporter|Salicylate reporter]] -- a system for Shewanella detecting salicylate and activating the [[Team:Cornell/Project/Mtr pathway|Mtr pathway]] to produce an electrical current | *[[Team:Cornell/Notebook/Salicylate reporter|Salicylate reporter]] -- a system for Shewanella detecting salicylate and activating the [[Team:Cornell/Project/Mtr pathway|Mtr pathway]] to produce an electrical current | ||
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| + | ==Protocols== | ||
| + | *[[Team:Cornell/Notebook/PCR|PCR]] | ||
| + | *[[Team:Cornell/Notebook/Gel_electrophoresis|Gel Electrophoresis]] | ||
| + | *[[Team:Cornell/Notebook/Gel_purification|Gel Purification]] | ||
| + | *[[Team:Cornell/Notebook/DNA_quatification|DNA Quantification]] | ||
| + | *[[Team:Cornell/Notebook/Digestion|Digestion Reaction]] | ||
| + | *[[Team:Cornell/Notebook/Dephosphorylation|Dephosphorylation]] | ||
| + | *[[Team:Cornell/Notebook/PCR_cleanup|PCR Clean Up]] | ||
| + | *[[Team:Cornell/Notebook/Ligation|Ligation Reaction]] | ||
| + | *[[Team:Cornell/Notebook/Transformation_ecoli|Transformation of E. coli]] | ||
| + | *[[Team:Cornell/Notebook/Transformation_ shewanella|Transformation of Shewanella]] | ||
| + | *[[Team:Cornell/Notebook/Sequencing|Sequencing]] | ||
Revision as of 21:26, 3 July 2012
| Home | Team | Project | Parts | Modeling | Notebook | Protocols | Safety | Attributions |
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Subprojects
- Arsenic reporter -- test a system for Shewanella detecting arsenates and activating the Mtr pathway to produce an electrical current
- nah operon -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter.
- Salicylate reporter -- a system for Shewanella detecting salicylate and activating the Mtr pathway to produce an electrical current
Protocols
- PCR
- Gel Electrophoresis
- Gel Purification
- DNA Quantification
- Digestion Reaction
- Dephosphorylation
- PCR Clean Up
- Ligation Reaction
- Transformation of E. coli
- Transformation of Shewanella
- Sequencing
