Team:EPF-Lausanne/Modeling

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= Introduction =
 
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== How LovTAP is thought to work ==
 
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=== Allosteric regulation ===
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To better understand the possibilities of this project, we've modeled different aspects of the protein and the elements around it. Please select the subject you want to know more about:
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In a protein, generally an enzyme, an allosteric site is any part of the protein other than the active site.
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*[[Team:EPF-Lausanne/Modeling/3D_structure|3D structure of the new fusion protein LovTAP-VP16]]
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Why and how we think the LovTAP-VP16 protein works?
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*[[Team:EPF-Lausanne/Modeling/Photoactivation|Photoactivation]]
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What proportion of  LovTAP-VP16 should we expect to be active depending on the lightning pattern?
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*[[Team:EPF-Lausanne/Modeling/Expression|Expression levels]]
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Getting a rough idea of the expression levels of reporter protein we can expect.
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*[[Team:EPF-Lausanne/Modeling/BioreactorSim|Bioreactor - Simulator]]
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Simulating the bioreactor lighting will give us an idea of the scalability of this type of production method.
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*[[Team:EPF-Lausanne/Bioreactor| Bioreactor - Assembly ]]
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We built our lighting device from scratch. Here are a few pictures of that process.
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[[File:Team-EPF-Lausanne-LovTAPmodel.png|400px|right]]
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Allosteric regulation of a protein consists in modifying its properties by interacting with an allosteric site.
 
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One example would be the regulation in the tryptophan (trp) operon,
 
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a group of genes studied in E. coli that are required for the synthesis of the amino acid tryptophan.
 
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The expression of these genes can be blocked by the homodimeric protein tryptophan repressor
 
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(TrpR), by binding the operator of the operon. TrpR repressing function is only active when
 
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tryptophan is bound to its allosteric sites, i.e. it blocks the production of tryptophan when the
 
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concentration of tryptophan is high.
 
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Latest revision as of 04:00, 27 September 2012


To better understand the possibilities of this project, we've modeled different aspects of the protein and the elements around it. Please select the subject you want to know more about:

Why and how we think the LovTAP-VP16 protein works?

What proportion of LovTAP-VP16 should we expect to be active depending on the lightning pattern?

Getting a rough idea of the expression levels of reporter protein we can expect.

Simulating the bioreactor lighting will give us an idea of the scalability of this type of production method.

We built our lighting device from scratch. Here are a few pictures of that process.


Team-EPF-Lausanne-LovTAPmodel.png