Team:Tokyo Tech/Parts

From 2012.igem.org

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<div class="whitebox">
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__NOTOC__
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<div id="tokyotech" style=" font:bold ;left ; font-size: 50px; color: #1E90FF; padding: 10px;">
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PARTS</div>
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<div class="whitebox">
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<div id="tokyotech" style=" font:bold ;left ; font-size: 15px; color: #000000; padding: 30px;">
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=Parts=
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For a brief overview of our main results, please have a look at our Main Results page. [[https://2012.igem.org/Team:Tokyo_Tech Go to "HOME"]]
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<div id="tokyotech" style=" font:bold ;left ; font-size: 30px; color: #1E90FF; padding: 10px;">
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Favorite Tokyo_Tech 2012 iGEM Team Parts</div>
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{| class="wikitable" cellpadding="6"
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| align="center" style="background:#f0f0f0;"|
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| align="center" style="background:#f0f0f0;"|'''Name'''
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| align="center" style="background:#f0f0f0;"|'''Type'''
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| align="center" style="background:#f0f0f0;"|'''Description'''
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| align="center" style="background:#f0f0f0;"|'''Designer'''
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| align="center" style="background:#f0f0f0;"|'''Length'''
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|-
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]||Composite||phaC1-A-B1 [P(3HB) synthesis]||Taku Nakayama||4208
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|-
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]||Composite||Plas-LuxI||Toshiki Hashimoto||755
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|-
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]||Composite||Plux-LasI||Toshiki Hashimoto||798
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|}
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<div id="tokyotech" style=" font:bold ;left ; font-size: 30px; color: #1E90FF; padding: 10px;">
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Tokyo_Tech 2012 iGEM Team Parts</div>
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<br><br><br><br>
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{| class="wikitable" cellpadding="6"
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| align="center" style="background:#f0f0f0;"|
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| align="center" style="background:#f0f0f0;"|'''Name'''
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| align="center" style="background:#f0f0f0;"|'''Type'''
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| align="center" style="background:#f0f0f0;"|'''Description'''
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| align="center" style="background:#f0f0f0;"|'''Designer'''
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| align="center" style="background:#f0f0f0;"|'''Length'''
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|-
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016]||Composite||Plas-LacI||Naoki Tsukuda||1332
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|-
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]||Regulatory||Plux/tet hybrid promoter||Toshiki Hashimoto||72
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|-
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]||Reporter||Plux/tet-GFP||Toshiki Hashimoto||955
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|-
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026]||Composite||Plux-LacI||Naoki Tsukuda||1304
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|}
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=Best new BioBrick part (natural): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]=
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We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).
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=Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]=
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<div id="tokyotech" style=" font:bold ;left ; font-size: 25px; color: #FF0000; padding: 10px;">
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(+BBa_K934012 is positive feedback system)</div>
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We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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=Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]=
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]:
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By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied.  
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Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.  
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]:
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By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.
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</div>
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</div>
<groupparts>iGEM012 Tokyo_Tech</groupparts>
<groupparts>iGEM012 Tokyo_Tech</groupparts>

Latest revision as of 03:52, 27 September 2012

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Tokyotechlogo2012.png

PARTS

Parts

For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]


Favorite Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]CompositephaC1-A-B1 [P(3HB) synthesis]Taku Nakayama4208
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]CompositePlas-LuxIToshiki Hashimoto755
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]CompositePlux-LasIToshiki Hashimoto798
Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016]CompositePlas-LacINaoki Tsukuda1332
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]RegulatoryPlux/tet hybrid promoterToshiki Hashimoto72
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]ReporterPlux/tet-GFPToshiki Hashimoto955
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026]CompositePlux-LacINaoki Tsukuda1304

Best new BioBrick part (natural): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]

We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).

Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]

(+BBa_K934012 is positive feedback system)

We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.

Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.

<groupparts>iGEM012 Tokyo_Tech</groupparts>

Retrieved from "http://2012.igem.org/Team:Tokyo_Tech/Parts"