Team:SEU A/Experiment/proof2
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- | <h1> | + | <h1>Sweet gene</h1> |
- | <span> | + | <span>The confirmation experiment of Sweet gene.</span> |
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<a href="https://2012.igem.org/Team:SEU_A/Experiment/proof4"><img src='https://static.igem.org/mediawiki/2012/8/8a/Seua_exp_death.png' width='200' height='200'></img></a> | <a href="https://2012.igem.org/Team:SEU_A/Experiment/proof4"><img src='https://static.igem.org/mediawiki/2012/8/8a/Seua_exp_death.png' width='200' height='200'></img></a> | ||
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+ | <h2>The confirmation experiment of Sweet gene.</h2></br> | ||
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+ | </html>[[Media:The confirmation experiment of Sweet gene.pdf |The confirmation experiment of Sweet gene.pdf]]<html> | ||
We get the Sweet gene groE which could help E-coli folding the protein correctly at high temperature from the genome of BL21. Caring this protein would make the host bacteria grow better at high temperature.</br> | We get the Sweet gene groE which could help E-coli folding the protein correctly at high temperature from the genome of BL21. Caring this protein would make the host bacteria grow better at high temperature.</br> | ||
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+ | <img src='https://static.igem.org/mediawiki/2012/8/8d/Sweet_gene1.jpg'></img> | ||
Experimental procedure:</br> | Experimental procedure:</br> | ||
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Step 4: add 5ul bacterial liquid which has been diluted times to liquid Luria-Bertani medium .Set temperature control. After cultivating for 12 to 16h, measure the OD600 of bacterial liquid.</br></br> | Step 4: add 5ul bacterial liquid which has been diluted times to liquid Luria-Bertani medium .Set temperature control. After cultivating for 12 to 16h, measure the OD600 of bacterial liquid.</br></br> | ||
+ | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/f/f9/Sweet_gene_Fig.1.jpg/800px-Sweet_gene_Fig.1.jpg'></img></br></center> | ||
Fig1. The experimental procedure of Sweet gene’s confirmatory experiment | Fig1. The experimental procedure of Sweet gene’s confirmatory experiment | ||
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The data of Sweet gene’s confirmation experiment version 1.0</br> | The data of Sweet gene’s confirmation experiment version 1.0</br> | ||
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Fig2. The initial OD600 of the groE and Blank | Fig2. The initial OD600 of the groE and Blank | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/2/2b/Sweet_gene.Fig3.jpg/800px-Sweet_gene.Fig3.jpg'></img></br></center> | |
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Fig3. The OD600 of the E.coli after 16h | Fig3. The OD600 of the E.coli after 16h | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/9/9a/Sweet_gene.Fig4.jpg/800px-Sweet_gene.Fig4.jpg'></img></br></center> | |
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Fig4. The average value of the OD600 of fig 2 | Fig4. The average value of the OD600 of fig 2 | ||
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The data of Sweet gene’s confirmation experiment version 2.0 | The data of Sweet gene’s confirmation experiment version 2.0 | ||
The initial OD600 of the g+p ( groE on the backbone of pET32a) and p( plasmid pET32a) | The initial OD600 of the g+p ( groE on the backbone of pET32a) and p( plasmid pET32a) | ||
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+ | <center></br><img src='https://static.igem.org/mediawiki/2012/c/ca/Seua_Sweet_gene.5.jpg'></img></br></center> | ||
After 4 h, we measure the OD600 of each kind of cell liquid:</br> | After 4 h, we measure the OD600 of each kind of cell liquid:</br> | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/8/83/Sweet_gene.6.jpg/600px-Sweet_gene.6.jpg'></img></br></center> | |
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Dealing with these data, we got the results as follows: | Dealing with these data, we got the results as follows: | ||
+ | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/b/bd/Sweet_gene.7.jpg/569px-Sweet_gene.7.jpg'></img></br></center> | ||
+ | Averaging the different volume, we have a more visual figure. The Sweet gene plays a powerful role in the temperature struggle.</br> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/7/77/Sweet_gene.8.jpg'></img> | ||
+ | After 17 hours:</br> | ||
+ | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/c/c0/Sweet_gene.9.jpg/600px-Sweet_gene.9.jpg'></img></br></center> | ||
+ | The average of data after 17h:</br> | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/b/bc/Sweet_gene.10.jpg/504px-Sweet_gene.10.jpg'></img></br></center> | |
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After 17h, we found a strange phenomenon. The g+p lives better at 42℃ without IPTG, and we don’t know the reason. Besides, the Sweet gene still works in a temperature struggle. | After 17h, we found a strange phenomenon. The g+p lives better at 42℃ without IPTG, and we don’t know the reason. Besides, the Sweet gene still works in a temperature struggle. | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/4/43/Sweet_gene.11.jpg'></img></br></center> | |
After 45h: | After 45h: | ||
- | + | <center></br> <img src='https://static.igem.org/mediawiki/2012/thumb/b/ba/Sweet_gene.12.jpg/605px-Sweet_gene.12.jpg'></img></br></center> | |
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+ | The average of data after 45h: | ||
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+ | <center></br><img src='https://static.igem.org/mediawiki/2012/thumb/d/d4/Sweet_gene.13.jpg/542px-Sweet_gene.13.jpg'></img></br></center> | ||
- | + | After 45h, the strange phenomenon became stranger. There was no regularity anymore. The Sweet gene was no longer performing its function. | |
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- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/b/b0/Sweet_gene.14.jpg'></img></br></center> | |
We still compared the OD600 of one type of bacteria while time elapses. | We still compared the OD600 of one type of bacteria while time elapses. | ||
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The volume of bacterial liquid is 5 ul. | The volume of bacterial liquid is 5 ul. | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/3/34/Sweet_gene.15.jpg'></img></br></center> | |
The volume of bacterial liquid is 10 ul. | The volume of bacterial liquid is 10 ul. | ||
- | + | <center></br><img src='https://static.igem.org/mediawiki/2012/e/e8/Sweet_gene.16.jpg'></img></br></center> | |
The volume of bacterial liquid is 15 ul. | The volume of bacterial liquid is 15 ul. | ||
+ | <center></br><img src='https://static.igem.org/mediawiki/2012/d/db/Sweet_gene.17.jpg'></img></br></center> | ||
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From these figures, we still got the same result as the data above. | From these figures, we still got the same result as the data above. | ||
Latest revision as of 03:48, 27 September 2012
![](https://static.igem.org/mediawiki/2012/a/af/Seua_title.png)
Sweet gene
The confirmation experiment of Sweet gene.![](https://static.igem.org/mediawiki/2012/3/3f/Seua_exp_HSP.png)
![](https://static.igem.org/mediawiki/2012/1/1f/Seua_exp_sweet_gene.png)
![](https://static.igem.org/mediawiki/2012/5/5b/Seua_exp_conjugation.png)
![](https://static.igem.org/mediawiki/2012/8/8a/Seua_exp_death.png)
The confirmation experiment of Sweet gene.
The confirmation experiment of Sweet gene.pdf We get the Sweet gene groE which could help E-coli folding the protein correctly at high temperature from the genome of BL21. Caring this protein would make the host bacteria grow better at high temperature.![](https://static.igem.org/mediawiki/2012/8/8d/Sweet_gene1.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/f/f9/Sweet_gene_Fig.1.jpg/800px-Sweet_gene_Fig.1.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/b/bb/Sweet_gene.Fig2.jpg/800px-Sweet_gene.Fig2.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/2/2b/Sweet_gene.Fig3.jpg/800px-Sweet_gene.Fig3.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/9/9a/Sweet_gene.Fig4.jpg/800px-Sweet_gene.Fig4.jpg)
![](https://static.igem.org/mediawiki/2012/c/ca/Seua_Sweet_gene.5.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/8/83/Sweet_gene.6.jpg/600px-Sweet_gene.6.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/b/bd/Sweet_gene.7.jpg/569px-Sweet_gene.7.jpg)
![](https://static.igem.org/mediawiki/2012/7/77/Sweet_gene.8.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/c/c0/Sweet_gene.9.jpg/600px-Sweet_gene.9.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/b/bc/Sweet_gene.10.jpg/504px-Sweet_gene.10.jpg)
![](https://static.igem.org/mediawiki/2012/4/43/Sweet_gene.11.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/b/ba/Sweet_gene.12.jpg/605px-Sweet_gene.12.jpg)
![](https://static.igem.org/mediawiki/2012/thumb/d/d4/Sweet_gene.13.jpg/542px-Sweet_gene.13.jpg)
![](https://static.igem.org/mediawiki/2012/b/b0/Sweet_gene.14.jpg)
![](https://static.igem.org/mediawiki/2012/3/34/Sweet_gene.15.jpg)
![](https://static.igem.org/mediawiki/2012/e/e8/Sweet_gene.16.jpg)
![](https://static.igem.org/mediawiki/2012/d/db/Sweet_gene.17.jpg)
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