Team:Tokyo-NoKoGen/Notebook/diary

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Template:Team:Tokyo-NoKoGen/contents_header}} {{Template:Team:Tokyo-NoKoGen/index_notebook}} <html> </html> {{Template:Team:Tokyo-NoKoGen/contents_footer}}")
 
(8 intermediate revisions not shown)
Line 2: Line 2:
{{Template:Team:Tokyo-NoKoGen/index_notebook}}
{{Template:Team:Tokyo-NoKoGen/index_notebook}}
<html>
<html>
 +
<BR>
 +
<BR>
 +
<font size=32>Diary</font>
 +
 +
<BR>
 +
<BR>
 +
<h2>July</h2>
 +
<h3>8-14</h3>
 +
Meeting :<BR>
 +
lux team member: Jinhee LEE, Sumiya KAWAI, Miho NARITA, Tomomi YOKOYAMA, Mayumi NAKAMURA <BR>
 +
rhodopsin team member: Hiroto OKANO, Genki SAKAI, Hayato TSURUTA, Somin LEE
 +
<BR>
 +
<BR>
 +
 +
<h2>August</h2>
 +
<h3>5-11</h3>
 +
Meeting : <BR>
 +
Small session on gene experiments using iGEM biobrick.<BR><BR>
 +
Lux team :<BR>
 +
Design primers to clone lux operon from photobacterim phosphorium genome DNA.
 +
<BR>
 +
<BR>
 +
 +
<h3>19-25</h3>
 +
Rhodopsin team :<BR>
 +
construction of blue light sensor.<BR>
 +
Pconst.(H)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-DT<BR>
 +
sequence analysis of constructed blue light sensor biobrick.<BR><BR>
 +
Meeting : <BR>
 +
lux team and rhodopsin team made small presentation about experiments.
 +
 +
<BR><BR>
 +
 +
<h3>26-9/1</h3>
 +
Rhodopsin team :<BR>
 +
construction of ⊿EnvZ competent cell<BR><BR>
 +
Meeting :<BR>
 +
Our project title and team character was desided! <BR>
 +
“Coli express for long distance communication”<BR><BR>
 +
Lux team :<BR>
 +
lux operon was cloned from <I>Photobacterim phosphorium</I> genome DNA. Most sequence was confirmed by sequence analysis.
 +
<br><br>
 +
<div align=center><img src="https://static.igem.org/mediawiki/2012/f/f8/Coli3.jpg" height="50%" width="65%"/></div>
 +
 +
<BR><BR>
 +
 +
<h3>2-8</h3>
 +
Meeting :<BR>
 +
lux team and rhodopsin team made small presentation about experiments.<BR><BR>
 +
Rhodopsin team :<BR>
 +
insertion of PompR(+)-RBS-GFP-DT under blue light sensor biobrick.(BBa_K769000)<BR>
 +
plasmid extraction of blue light sensor + GFP and sequence analysis.
 +
 +
<BR><BR>
 +
 +
<h3>9-15</h3>
 +
Lux team : <BR>
 +
lux bio-brick (pSB1C3-PBAD-RBS-lux operon-DT) was constructed! <I>E. coli</I> TOP10 was transformed with this plasmid.<BR>
 +
Evaluation of lux bio-brick. Luminescence was measured using plate reader. We could see luminescence in dark room.<BR>
 +
Design primers to clone rib operon from <I>E.coli</I> genome DNA<BR>
 +
lux bio-brick that constitutive expression of lux gene was constructed. We expected more luminescence using strong constitutive expression promoter.<BR>
 +
<BR>
 +
Rhodopsin team :<BR>
 +
evaluation of blue light sensor.<BR>
 +
After preculture, transformants were culture under blue light or dark condition.<BR>
 +
But cells were not growth well.<BR><BR>
 +
Meeting :<BR>
 +
Our team T-shirt design idea has been completed.<BR>
 +
<div align=center><img src="https://static.igem.org/mediawiki/2012/0/02/Tshirt.png"></div>
 +
<BR><BR>
 +
 +
<h3>16-22</h3>
 +
Lux team :<BR>
 +
rib operon was cloned from <I>E.coli</I> genome DNA. Most sequence was confirmed by sequence analysis<BR>
 +
Evaluation of color change biobrick.<BR><BR>
 +
Meeting :<BR><BR>
 +
Presentation team <BR>
 +
Poster session team<BR>
 +
We began to design poster and slide.<BR>
 +
<div align=center><img src="https://static.igem.org/mediawiki/2012/0/02/Col.png"></div>
 +
<BR><BR>
 +
 +
<h3>23-29</h3>
 +
Rhodopsin team : <BR>
 +
Reevaluation of blue light sensor.<BR>
 +
After preculture, transformants were culture under white light or dark condition.<BR><BR>
 +
Lux team :<BR>
 +
evaluation of fast luminescence biobrick.
 +
<br>
 +
<div align=center><img src="https://static.igem.org/mediawiki/2012/5/59/Fast.png"></div>
 +
 +
<BR><BR>

Latest revision as of 03:39, 27 September 2012



Diary

July

8-14

Meeting :
lux team member: Jinhee LEE, Sumiya KAWAI, Miho NARITA, Tomomi YOKOYAMA, Mayumi NAKAMURA
rhodopsin team member: Hiroto OKANO, Genki SAKAI, Hayato TSURUTA, Somin LEE

August

5-11

Meeting :
Small session on gene experiments using iGEM biobrick.

Lux team :
Design primers to clone lux operon from photobacterim phosphorium genome DNA.

19-25

Rhodopsin team :
construction of blue light sensor.
Pconst.(H)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-DT
sequence analysis of constructed blue light sensor biobrick.

Meeting :
lux team and rhodopsin team made small presentation about experiments.

26-9/1

Rhodopsin team :
construction of ⊿EnvZ competent cell

Meeting :
Our project title and team character was desided!
“Coli express for long distance communication”

Lux team :
lux operon was cloned from Photobacterim phosphorium genome DNA. Most sequence was confirmed by sequence analysis.



2-8

Meeting :
lux team and rhodopsin team made small presentation about experiments.

Rhodopsin team :
insertion of PompR(+)-RBS-GFP-DT under blue light sensor biobrick.(BBa_K769000)
plasmid extraction of blue light sensor + GFP and sequence analysis.

9-15

Lux team :
lux bio-brick (pSB1C3-PBAD-RBS-lux operon-DT) was constructed! E. coli TOP10 was transformed with this plasmid.
Evaluation of lux bio-brick. Luminescence was measured using plate reader. We could see luminescence in dark room.
Design primers to clone rib operon from E.coli genome DNA
lux bio-brick that constitutive expression of lux gene was constructed. We expected more luminescence using strong constitutive expression promoter.

Rhodopsin team :
evaluation of blue light sensor.
After preculture, transformants were culture under blue light or dark condition.
But cells were not growth well.

Meeting :
Our team T-shirt design idea has been completed.


16-22

Lux team :
rib operon was cloned from E.coli genome DNA. Most sequence was confirmed by sequence analysis
Evaluation of color change biobrick.

Meeting :

Presentation team
Poster session team
We began to design poster and slide.


23-29

Rhodopsin team :
Reevaluation of blue light sensor.
After preculture, transformants were culture under white light or dark condition.

Lux team :
evaluation of fast luminescence biobrick.


Retrieved from "http://2012.igem.org/Team:Tokyo-NoKoGen/Notebook/diary"