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Team:Uppsala University
From 2012.igem.org
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Constructed smallRNA downregulating antibiotic resistance | Constructed smallRNA downregulating antibiotic resistance | ||
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Revision as of 03:00, 27 September 2012
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Project descriptionTeam Uppsala University 2012 is dedicated to combating the rising antibiotic resistance in bacteria by means of synthetic biology. Old and well-known antibiotics are quickly becoming ineffective as resistance genes are spreading. Scientist around the world struggle with varying success to develop new antibacterial substances. But do we really have to abandon classic antibiotics? Team Uppsala University begs to differ, we believe new methods will allow us to combat the resistance itself, and make the bacteria once again sensitive to old drugs. Working with real-world resistance genes isolated from ESBL outbreaks at Swedish hospitals, we are developing anti-resistance systems active at three different levels: DNA level, transcriptional level and translational level. Our systems will be delivered to the target bacteria using an engineered phage and/or a conjugative plasmid. At DNA level, we will develop a method for permanent removal of plasmids from bacteria. Using TAL Effector Nucleases, we will be able to target and cut individual resistance genes. At transcriptional level, we will use synthetic super-repressors to repress transcription of resistance genes and native defense mechanisms in bacteria. At translational level, we will construct a modular large-scale screening system for sRNA:s and use it to find strongly silencing RNA sequences against three common resistance genes. With this team on the project, there is no question about it: Resistance is futile! |
Achivements
SmallRNA working!
Constructed smallRNA downregulating antibiotic resistance Improved existing part Improved standard plasmid backbones from the 4 series. Cool new biobricks Made several biobricks and new applications for them, demonstrated how they worked and characterized them. Helped other teams. By sending several of oour constructed biobrick parts to other teams. Characterization of promotors Measured several different promotors to gain better understand of promotor choice. |
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The projectIn this project, Team Uppsala University will attempt to counteract the molecular machinery making a bacterium resistant to antibiotics. Using three separate methods, our hope is to silence the genes responsible for resistance accurately and effectively. |
The universityFounded in 1477, Uppsala University is the oldest and one of the most highly regarded universities in Sweden. Throughout the years, notable people such as Carl Linnaeus and Anders Celsius have been professors at the university. |
The competitionThe International Genetically Engineered Machine (iGEM) competition is a worldwide event hosted by MIT surrounding the area of Synthetic Biology. Once a year teams from all over the world design and execute their own projects, hoping to win the prestigious first prize. |
