Team:CD-SCU-CHINA

From 2012.igem.org

(Difference between revisions)
 
(27 intermediate revisions not shown)
Line 1: Line 1:
-
<!--DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"-->
+
{{SCU-IGEM}}
-
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr">
+
*Experiment
-
 
+
Oil pollution is an increasingly grave environmental problem. Artificial hydrocarbonoclastic bacterium plays a critical role in the bioremediation of oil polluted marine systems.<br>
-
<head>
+
Since this problem has long been tricky and not solved yet, we plan to construct a complex system to degrade oil spill in Escherichia coil, which mainly consists of 3 circuits: degradation circuit, emulsification circuit and stress tolerance circuit. Moreover, there are some other assistant circuits, RBS optimizing and subsequent dehydrogenation for example.<br>
-
      <meta http-equiv="content-type" content="text/html; charset=UTF-8"/>
+
More details please see the [[Project]]<br>
-
<meta name="description" content=""/>
+
In the degradatiom circuit, we studyed two gene involes in it, P450, a cytochrome, which responsible for the medium-chain-length chain and long chain hydrocarbone. AlkB2, a Terminal Oxidase, which oxidate the hydroxy to carboxyl.<br>
-
<meta name="keywords" content="" />
+
In the emulsification circuit, one gene OPRF which encode one enzyme, that can produce the glycolipid for the emucification of the oil spill.<br>
-
<meta name="author" content="" />
+
In the sensing circuit, we found to two gene, Alks, which encodes one transcriptional factor, can sense the existence of hydrocarbon, then bind directly to the induction promoter, and the Gnt, also one transcriptional factor, can sense the level of AMP, in our project, we want to have one circuits that can sense the low energy state from saccharides, and turn on the state of using hydrocabone. <br>
-
<style type="text/css">
+
Our plane just change the forepart of the normal glucose degration sysyterm, use an hydrocarbone degradation instead, but the dowstream proceed are the same. <br>
-
/*
+
*'''Sponsors:'''
-
  Reset
+
[[File:SCU-IGEM_SPONECOR2.png|400px|right bottom]]
-
------------------------------------------------------------------- */
+
[[File:SCU-IGEM_SPONECOR1.jpg|360px|left bottom]]
-
 
+
-
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, p, blockquote, pre, a, abbr, acronym, address, code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td, textarea, input, select {margin: 0; padding: 0; border: 0; font-weight: inherit; font-style: inherit; font-size: 100%; font-family: inherit; vertical-align: baseline;}
+
-
table {border-collapse: collapse; border-spacing: 0;}
+
-
caption, th, td {text-align: left; font-weight: normal;}
+
-
table, td, th {vertical-align: middle;}
+
-
blockquote:before, blockquote:after, q:before, q:after {content: "";}
+
-
blockquote, q {quotes: "" "";}
+
-
a img {border: none;}
+
-
:focus {outline: 0;}
+
-
 
+
-
 
+
-
/*  
+
-
  General
+
-
------------------------------------------------------------------- */
+
-
 
+
-
html {
+
-
height: 100%;
+
-
padding-bottom: 1px; /* force scrollbars */
+
-
}
+
-
 
+
-
body {
+
-
background: #A9A9A9;
+
-
color: #444;
+
-
font: normal 75% sans-serif;
+
-
line-height: 1.5;
+
-
}
+
-
 
+
-
 
+
-
/*
+
-
  Typography
+
-
------------------------------------------------------------------- */
+
-
 
+
-
/* Headings */
+
-
 
+
-
h1,h2,h3,h4,h5,h6 {
+
-
color: #444;
+
-
font-weight: normal;
+
-
line-height: 1;
+
-
margin-bottom: 0.3em;
+
-
}
+
-
h4,h5,h6 {font-weight: bold;}
+
-
 
+
-
h1 {font-size: 2.6em;}
+
-
h2 {font-size: 2em;}
+
-
h3 {font-size: 1.5em;}
+
-
h4 {font-size: 1.25em;}
+
-
h5 {font-size: 1.1em;}
+
-
h6 {font-size: 1em;}
+
-
 
+
-
h1 img, h2 img, h3 img, h4 img, h5 img, h6 img {margin: 0;}
+
-
 
+
-
 
+
-
/* Text elements */
+
-
 
+
-
p {margin-bottom: 1em;}
+
-
 
+
-
a:focus,a:hover {color: #002;}
+
-
a {
+
-
color: #246;
+
-
text-decoration: underline;
+
-
}
+
-
 
+
-
abbr, acronym {border-bottom: 1px dotted #666;}
+
-
address {margin-bottom: 1.5em;}
+
-
blockquote {margin: 1.2em; line-height: 1.4em;}
+
-
blockquote span {font-size: 1.4em; color: #000;}
+
-
del {color:#666;}
+
-
em, dfn, blockquote, address {font-style: italic;}
+
-
strong, dfn {font-weight: bold;}
+
-
sup, sub {line-height: 0;}
+
-
cite {color: #666;}
+
-
 
+
-
pre {
+
-
margin: 1.5em 0;
+
-
white-space: pre;
+
-
}
+
-
pre,code,tt {
+
-
font: 1em monospace;
+
-
line-height: 1.5;
+
-
}
+
-
 
+
-
 
+
-
/* Lists */
+
-
 
+
-
li ul, li ol {margin-left: 1.5em;}
+
-
ul, ol {margin: 0 1.5em 1.5em 1.5em;}
+
-
 
+
-
ul {list-style-type: disc;}
+
-
ol {list-style-type: decimal;}
+
-
 
+
-
dl {margin: 0 0 1.5em 0;}
+
-
dl dt {font-weight: bold;}
+
-
dd {margin-left: 1.5em;}
+
-
 
+
-
 
+
-
/* Tables */
+
-
 
+
-
table {margin-bottom: 1.4em; width: 100%;}
+
-
th {font-weight: bold;}
+
-
thead th {background: #C3D9FF;}
+
-
th,td,caption {padding: 4px 10px 4px 5px;}
+
-
tr.even td {background: #F2F6FA;}
+
-
tfoot {font-style: italic;}
+
-
caption {background: #EEE;}
+
-
 
+
-
table.data-table {
+
-
border: 1px solid #EEE;
+
-
margin-bottom: 2em;
+
-
width: 100%;
+
-
}
+
-
table.data-table th {
+
-
background: #EEE;
+
-
border: 1px solid #DDD;
+
-
color: #555;
+
-
text-align: left;
+
-
}
+
-
table.data-table tr {border-bottom: 1px solid #EEE;}
+
-
table.data-table td, table th {padding: 8px 10px;}
+
-
table.data-table td {
+
-
background: #F6F6F6;
+
-
border: 1px solid #E6E6E6;
+
-
}
+
-
table.data-table tr.even td {background: #FCFCFC;}
+
-
 
+
-
 
+
-
/* Misc classes */
+
-
 
+
-
.small {font-size: 0.9em;}
+
-
.smaller {font-size: 0.8em;}
+
-
.smallest {font-size: 0.7em;}
+
-
 
+
-
.large {font-size: 1.1em;}
+
-
.larger {font-size: 1.2em;}
+
-
.largest {font-size: 1.3em;}
+
-
 
+
-
.hidden {display: none;}
+
-
 
+
-
.quiet {color: #666;}
+
-
.loud {color: #000;}
+
-
.highlight {background: #FF0;}
+
-
 
+
-
.text-left {text-align: left;}
+
-
.text-right {text-align: right;}
+
-
.text-center {text-align: center;}
+
-
 
+
-
.error, .notice, .success {
+
-
border: 1px solid #DDD;
+
-
margin-bottom: 1em;
+
-
padding: 0.6em 0.8em;
+
-
}
+
-
 
+
-
.error {background: #FBE3E4; color: #8A1F11; border-color: #FBC2C4;}
+
-
.error a {color: #8A1F11;}
+
-
 
+
-
.notice {background: #FFF6BF; color: #514721; border-color: #FFD324;}
+
-
.notice a {color: #514721;}
+
-
 
+
-
.success {background: #E6EFC2; color: #264409; border-color: #C6D880;}
+
-
.success a {color: #264409;}
+
-
 
+
-
div.more {padding-top: 6px;}
+
-
.more a, a.more {color: #666;}
+
-
.more a:hover, a.more:hover {color: #002;}
+
-
 
+
-
 
+
-
/*
+
-
  Forms
+
-
------------------------------------------------------------------- */
+
-
 
+
-
label {font-weight: bold; cursor: pointer;}
+
-
fieldset {padding: 1.4em; margin: 0 0 1.5em 0; border: 1px solid #DDD; background: #F6F6F6;}
+
-
legend {font-weight: bold; font-size: 1.2em;}
+
-
textarea {overflow: auto;}
+
-
input.text, textarea, select {
+
-
background: #FCFCFC;
+
-
border: 1px inset #AAA;
+
-
margin: 0.5em 0;
+
-
padding: 4px 5px;
+
-
}
+
-
input.text:focus, textarea:focus, select:focus {background: #FFFFF5;}
+
-
 
+
-
input.button {
+
-
background: #DDD;
+
-
border: 1px outset #AAA;
+
-
padding: 4px 5px;
+
-
}
+
-
input.button:active {border-style: inset;}
+
-
 
+
-
.form-error {border-color: #F00;}
+
-
 
+
-
 
+
-
/*
+
-
  Alignment
+
-
------------------------------------------------------------------- */
+
-
 
+
-
/* General */
+
-
 
+
-
.center,.aligncenter {
+
-
display: block;
+
-
margin-left: auto;
+
-
margin-right: auto;
+
-
}
+
-
 
+
-
 
+
-
/* Images */
+
-
 
+
-
img.bordered,img.alignleft,img.alignright,img.aligncenter {
+
-
background-color: #FFF;
+
-
border: 1px solid #DDD;
+
-
padding: 3px;
+
-
}
+
-
img.alignleft, img.left {margin: 0 1.5em 1em 0;}
+
-
img.alignright, img.right {margin: 0 0 1em 1.5em;}
+
-
 
+
-
 
+
-
/* Floats */ 
+
-
 
+
-
.left,.alignleft {float: left;}
+
-
.right,.alignright {float: right;}
+
-
 
+
-
.clear,.clearer {clear: both;}
+
-
.clearer {
+
-
display: block;
+
-
font-size: 0;
+
-
line-height: 0;
+
-
}
+
-
 
+
-
 
+
-
/* Columns */
+
-
 
+
-
.col2 {width: 47%;}
+
-
 
+
-
 
+
-
 
+
-
/*
+
-
  Separators
+
-
------------------------------------------------------------------- */
+
-
 
+
-
.content-separator, .archive-separator {
+
-
background: #EEE;
+
-
clear: both;
+
-
color: #FFE;
+
-
display: block;
+
-
font-size: 0;
+
-
height: 1px;
+
-
line-height: 0;
+
-
margin: 12px 0 24px;
+
-
}
+
-
.archive-separator {margin: 0 0 14px;}
+
-
 
+
-
.separator-vertical {background: url('CD-SCU-CHINA separator-vertical.gif') repeat-y center top;}
+
-
 
+
-
 
+
-
/*
+
-
  Posts
+
-
------------------------------------------------------------------- */
+
-
 
+
-
.post {margin-bottom: 24px;}
+
-
 
+
-
.post a {text-decoration: none;}
+
-
.post a:hover, .post-body a {text-decoration: underline;}
+
-
 
+
-
.post-title {border-bottom: 1px solid #E5E5E5;}
+
-
 
+
-
.post-date {
+
-
color: #777;
+
-
font-size: 0.9em;
+
-
padding: 8px 0 0;
+
-
}
+
-
.post-date a {color: #444;}
+
-
 
+
-
.post-body {padding-top: 12px;}
+
-
 
+
-
.post-meta {
+
-
background: #FCFCFC;
+
-
border: 1px solid #ECECEC;
+
-
color: #777;
+
-
font-size: 0.9em;
+
-
padding: 6px 10px;
+
-
}
+
-
.post-meta a {color: #345; }
+
-
.post-meta a:hover {color: #001;}
+
-
 
+
-
 
+
-
ul.nice-list {
+
-
border-top: 1px solid #F0F0F0;
+
-
margin-left: 0;
+
-
margin-right: 0;
+
-
}
+
-
ul.nice-list li {
+
-
border-bottom: 1px solid #F0F0F0;
+
-
list-style: none;
+
-
padding: 6px 5px;
+
-
}
+
-
ul.nice-list li a {text-decoration: none;}
+
-
ul.nice-list li a:hover {text-decoration: underline;}
+
-
ul.nice-list span {color: #666;}
+
-
 
+
-
#sidebar ul.nice-list li,#sidebar ul.nice-list {border-color: #E0E0E0;}
+
-
 
+
-
 
+
-
/* Archives */
+
-
.archive-pagination {margin-bottom: 1.6em;}
+
-
.archive-pagination a {text-decoration: none;}
+
-
.archive-pagination a:hover {text-decoration: underline;}
+
-
.archive-post {margin-bottom: 14px;}
+
-
.archive-post-date {
+
-
background: #F5F5F5;
+
-
border-bottom: 1px solid #C5C5C5;
+
-
border-right: 1px solid #CFCFCF;
+
-
float: left;
+
-
margin-right: 12px;
+
-
padding: 2px 0 5px;
+
-
text-align: center;
+
-
width: 46px;
+
-
}
+
-
.archive-post .post-date {
+
-
border: none;
+
-
padding: 0;
+
-
}
+
-
.archive-post-day {font: normal 1.6em Georgia,serif;}
+
-
.archive-post .post-date,.archive-post-title {padding-top: 3px;}
+
-
 
+
-
 
+
-
/*  
+
-
  Thumbnails
+
-
------------------------------------------------------------------- */
+
-
 
+
-
.thumbnails {margin: 0 0 1em -8px;}
+
-
.thumbnails a.thumb {
+
-
background: #DAD6D0;
+
-
display: block;
+
-
float: left;
+
-
margin: 0 0 8px 8px;
+
-
padding: 1px;
+
-
}
+
-
.thumbnails a.thumb:hover {background: #AAA;}
+
-
.thumbnails .thumb img {display: block;}
+
-
 
+
-
 
+
-
/*
+
-
  Box
+
-
------------------------------------------------------------------- */
+
-
 
+
-
.box {
+
-
background: url('CD-SCU-CHINA box.gif') no-repeat left top;
+
-
border-bottom: 1px solid #DFDFDA;
+
-
border-top: 1px solid #FFF;
+
-
padding: 16px 20px 0;
+
-
}
+
-
.box a {color: #951;}
+
-
.box a:hover {color: #310;}
+
-
.box-title {
+
-
color: #665;
+
-
font: normal 1.6em sans-serif;
+
-
}
+
-
.box-content {padding: 8px 0;}
+
-
 
+
-
 
+
-
 
+
-
/*
+
-
  Comments
+
-
------------------------------------------------------------------- */
+
-
 
+
-
div.comment-list {
+
-
border-top: 1px solid #EEE;
+
-
margin: 1em 0 2em;
+
-
}
+
-
 
+
-
.comment {
+
-
border-bottom: 1px solid #EEE;
+
-
padding: 10px 8px 0;
+
-
}
+
-
.comment-date {font-size: 0.9em;}
+
-
.comment-date a {
+
-
color: #567;
+
-
text-decoration: none;
+
-
}
+
-
.comment-date a:hover {
+
-
color: #001;
+
-
text-decoration: underline;
+
-
}
+
-
.comment-body {padding-top: 4px;}
+
-
 
+
-
.comment-gravatar {width: 48px;}
+
-
.comment-gravatar img {
+
-
background: #FFF;
+
-
border: 1px solid #DDD;
+
-
padding: 2px;
+
-
}
+
-
 
+
-
 
+
-
/*
+
-
  Layout
+
-
------------------------------------------------------------------- */
+
-
 
+
-
/* Common */
+
-
.center-wrapper {
+
-
width: 960px;
+
-
margin: 0 auto;
+
-
}
+
-
 
+
-
/* Header */
+
-
 
+
-
#header-wrapper {background: #2F2F2F url('CD-SCU-CHINA header.gif') repeat-x left top;}
+
-
#header-wrapper-2 {background: url('CD-SCU-CHINA header-wrapper-2.gif') repeat-y center top;}
+
-
#header {
+
-
background: #333 url('CD-SCU-CHINA header.gif') repeat-x left top;
+
-
padding: 32px 32px 0 46px;
+
-
}
+
-
 
+
-
#help-wrapper {
+
-
background: url('CD-SCU-CHINA help.gif') no-repeat left top;
+
-
height: 39px;
+
-
margin: -8px 0 0 auto;
+
-
width: 290px;
+
-
}
+
-
#help {
+
-
color: #666;
+
-
font-size: 1.2em;
+
-
padding: 10px 28px 0 32px;
+
-
}
+
-
#help a {
+
-
color: #999;
+
-
text-decoration: none;
+
-
}
+
-
#help a:hover {color: #DDD;}
+
-
 
+
-
#help span.text-separator {
+
-
color: #444;
+
-
padding: 0 7px;
+
-
}
+
-
 
+
-
#logo {color: #FFF;}
+
-
#site-title a {text-decoration: none;}
+
-
#site-title, #site-title a {color: #FFF;}
+
-
#site-title span, #site-title a span {color: #DDD;}
+
-
#site-slogan {
+
-
color: #B58361;
+
-
font-size: 1.3em;
+
-
}
+
-
 
+
-
 
+
-
/* Navigation */
+
-
 
+
-
ul.tabbed {
+
-
display: inline;
+
-
margin: 0;
+
-
padding: 0;
+
-
}
+
-
ul.tabbed li {
+
-
float: left;
+
-
list-style: none;
+
-
}
+
-
ul.tabbed a {text-decoration: none;}
+
-
 
+
-
#navigation-wrapper {background: url('CD-SCU-CHINA navigation.gif') repeat-x left top;}
+
-
#navigation-wrapper-2 {background: url('CD-SCU-CHINA navigation-wrapper-2.gif') no-repeat center top;}
+
-
#navigation {
+
-
background: url('CD-SCU-CHINA navigation.gif') repeat-x left top;
+
-
min-height: 46px;
+
-
padding: 0 8px;
+
-
}
+
-
 
+
-
#navigation a {
+
-
color: #EAE6DD;
+
-
display: block;
+
-
font-weight: bold;
+
-
font-size: 1.3em;
+
-
padding: 12px 12px 11px;
+
-
}
+
-
#navigation a:hover {color: #FFF;}
+
-
#navigation li.current_page_item a, #navigation li.current_page_parent a {color: #FFE;}
+
-
#navigation li.current_page_item a {background: url('CD-SCU-CHINA navigation-arrow-2.gif') no-repeat center bottom;}
+
-
#navigation li.current_page_parent a {background: url('CD-SCU-CHINA navigation-arrow.gif') no-repeat center bottom;}
+
-
 
+
-
 
+
-
/* Subnav */
+
-
 
+
-
#subnav-wrapper {background: #E9E9E9 url('CD-SCU-CHINA subnav-wrapper.gif') repeat-x left bottom;}
+
-
#subnav-wrapper-2 {background: url('CD-SCU-CHINA subnav-wrapper-2.gif') no-repeat center bottom;}
+
-
#subnav {padding: 0 8px;}
+
-
 
+
-
#subnav a {
+
-
color: #666;
+
-
display: block;
+
-
font: bold 1.1em sans-serif;
+
-
padding: 12px 12px;
+
-
}
+
-
#subnav a:hover {color: #111;}
+
-
#subnav li.current_page_item a {color: #333;}
+
-
 
+
-
 
+
-
/* Content */
+
-
 
+
-
.content {font-size: 1.1em;}
+
-
#content-wrapper {background: #F6F6F6 url('CD-SCU-CHINA content-wrapper.gif') repeat-y center top;}
+
-
.content#content-two-columns {background: url('CD-SCU-CHINA content-two-columns.gif') repeat-y left top;}
+
-
 
+
-
 
+
-
/* Main */
+
-
 
+
-
#main-wrapper {
+
-
float: right;
+
-
width: 698px;
+
-
}
+
-
#main {padding: 24px;}
+
-
 
+
-
#main .text-separator, #featured .text-separator {
+
-
color: #AAA;
+
-
padding: 0 5px;
+
-
}
+
-
 
+
-
 
+
-
/* Sidebar */
+
-
 
+
-
#sidebar-wrapper {
+
-
float: left;
+
-
width: 260px;
+
-
}
+
-
 
+
-
 
+
-
/* Footer */
+
-
 
+
-
#footer-wrapper {
+
-
background: url('CD-SCU-CHINA footer-wrapper.gif') no-repeat center bottom;
+
-
border-top: 1px solid #999;
+
-
padding-bottom: 20px;
+
-
}
+
-
#footer {
+
-
background: #808080;
+
-
font-size: 1.1em;
+
-
padding: 12px 16px;
+
-
}
+
-
#footer a {
+
-
color: #DDD;
+
-
text-decoration: none;
+
-
}
+
-
#footer a:hover {color: #FFF;}
+
-
#footer span.text-separator {
+
-
color: #999;
+
-
padding: 0 3px;
+
-
}
+
-
 
+
-
/* Bottom */
+
-
 
+
-
#bottom {
+
-
color: #666;
+
-
padding: 0 6px 12px;
+
-
margin-top: -6px;
+
-
}
+
-
#bottom a {
+
-
color: #555;
+
-
text-decoration: none;
+
-
}
+
-
#bottom a:hover, #bottom .right a:hover {color: #002;}
+
-
#bottom span.text-separator {
+
-
color: #888;
+
-
padding: 0 3px;
+
-
}
+
-
#bottom .right, #bottom .right a {color: #777;}
+
-
 
+
-
 
+
-
/* Featured Box */
+
-
#featured-wrapper {
+
-
background: url('CD-SCU-CHINA gradient-light.gif') repeat-x left bottom;
+
-
border-bottom: 1px solid #E0E0E0;
+
-
padding-bottom: 22px;
+
-
}
+
-
#featured {padding: 42px 124px 12px 48px;}
+
-
 
+
-
#featured h2 {
+
-
font: normal 3em sans-serif;
+
-
margin: 0;
+
-
}
+
-
#featured h2 span {color: #43B16C;}
+
-
 
+
-
 
+
-
/*
+
-
  Misc overriding classes
+
-
------------------------------------------------------------------- */
+
-
 
+
-
/* Border */
+
-
 
+
-
.noborder {border: 0;}
+
-
.notborder {border-top: 0;}
+
-
.norborder {border-right: 0;}
+
-
.nobborder {border-bottom: 0;}
+
-
.nolborder {border-left: 0;}
+
-
 
+
-
/* Margin */
+
-
 
+
-
.nomargin {margin: 0;}
+
-
.notmargin {margin-top: 0;}
+
-
.normargin {margin-right: 0;}
+
-
.nobmargin {margin-bottom: 0;}
+
-
.nolmargin {margin-left: 0;}
+
-
 
+
-
/* Padding */
+
-
 
+
-
.nopadding {padding: 0;}
+
-
.notpadding {padding-top: 0;}
+
-
.norpadding {padding-right: 0;}
+
-
.nobpadding {padding-bottom: 0;}
+
-
.nolpadding {padding-left: 0;}
+
-
 
+
-
/* Single Line IE Fixes */
+
-
* html .separator-vertical, * html .content {height: 0.01%; min-height: 0.01%;}
+
-
</style>
+
-
 
+
-
</head>
+
-
 
+
-
<body id="top">
+
-
 
+
-
<div id="header-wrapper">
+
-
<div id="header-wrapper-2">
+
-
<div class="center-wrapper">
+
-
 
+
-
<div id="header">
+
-
 
+
-
<div id="logo">
+
-
<h1 id="site-title"><a href="#">2012 IGEM <span>SCU</span></a></h1>
+
-
<h2 id="site-slogan">&nbsp;</h2>
+
-
</div>
+
-
 
+
-
<div id="help-wrapper">
+
-
<div id="help"></div>
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id="navigation-wrapper">
+
-
<div id="navigation-wrapper-2">
+
-
<div class="center-wrapper">
+
-
+
-
<div id="navigation">
+
-
 
+
-
<ul class="tabbed">
+
-
<li class="current_page_item"><a href="index.html">Home</a></li>
+
-
<li><a href="Projects.html">Projects</a></li>
+
-
<li><a href="Team.html">Team</a></li>
+
-
<li><a href="Pars.html">Pars Submitted to the Registry</a></li>
+
-
<li><a href="Modeling.html">Modeling</a></li>
+
-
<li><a href="Notebook.html">Notebook</a></li>
+
-
                    <li><a href="Safty.html">Safty</a></li>
+
-
</ul>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
</div>
+
-
</div>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<div id="content-wrapper">
+
-
<div class="center-wrapper">
+
-
+
-
<div class="content">
+
-
 
+
-
<div id="featured-wrapper">
+
-
<div id="featured">
+
-
 
+
-
<div class="left" style="width: 330px">
+
-
+
-
<p><img [[File:CD-SCU-CHINA.jpg]] width="360" height="320" alt="" /></p>
+
-
 
+
-
 
+
-
</div>
+
-
 
+
-
<div class="right">
+
-
<img [[File:CD-SCU-CHINA logo.png]] width="360" height="320" alt="" />
+
-
</div>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id="main">
+
-
 
+
-
<div class="separator-vertical">
+
-
 
+
-
<div class="col2 left">
+
-
 
+
-
<h2 class="left">Our Projects</h2>
+
-
<div class="right more large"><a href="Projects.html">All Projects &#187;</a></div>
+
-
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
<div class="content-separator"></div>
+
-
 
+
-
<img src="sample-thumbnail.jpg" width="64" height="64" alt="" class="left bordered" />
+
-
 
+
-
<h3><a href="Projects.html">Project1</a></h3>
+
-
+
-
<p>Project1--content</p>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
<div class="content-separator"></div>
+
-
+
-
<img src="sample-thumbnail.jpg" width="64" height="64" alt="" class="left bordered" />
+
-
 
+
-
<h3><a href="Projects.html">Project2</a></h3>
+
-
+
-
<p>Project2--content</p>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
<div class="content-separator"></div>
+
-
 
+
-
<img src="sample-thumbnail.jpg" width="64" height="64" alt="" class="left bordered" />
+
-
 
+
-
<h3><a href="Projects.html">Project3</a></h3>
+
-
+
-
<p>Project3--content</p>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div class="col2 right">
+
-
 
+
-
<h2 class="left">Notebook</h2>
+
-
<div class="right more large"><a href="Notebook.html">View More &#187;</a></div>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
<ul class="nice-list">
+
-
<li><span>2012-07-01 |</span> <a href="Notebook.html">Notebook--content</a></li>
+
-
<li><span>2012-07-08 |</span> <a href="Notebook.html">Notebook--content</a></li>
+
-
<li><span>2012-07-15 |</span> <a href="Notebook.html">Notebook--content</a></li>
+
-
<li><span>2012-07-22 |</span> <a href="Notebook.html">Notebook--content</a></li>
+
-
<li><span>2012-07-29 |</span> <a href="Notebook.html">Notebook--content</a></li>
+
-
</ul>
+
-
+
-
<h2 class="left">Our Team</h2>
+
-
<div class="right more large"><a href="Team.html">View More &#187;</a></div>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
<blockquote>
+
-
<p><span>&quot;</span> content<span>&quot;</span></p>
+
-
<cite>content</cite>
+
-
</blockquote>
+
-
 
+
-
<blockquote>
+
-
<p><span>&quot;</span>content<span>&quot;</span></p>
+
-
<cite>content</cite>
+
-
</blockquote>
+
-
 
+
-
</div>
+
-
 
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id="footer-wrapper">
+
-
 
+
-
<div class="center-wrapper">
+
-
 
+
-
<div id="footer">
+
-
 
+
-
<div class="left">
+
-
<a href="index.html">Home</a> <span class="text-separator">|</span> <a href="Projects.html">Projects</a> <span class="text-separator">|</span> <a href="Team.html">Team</a> <span class="text-separator">|</span> <a href="Pars.html">Pars Submitted to the Registry</a> <span class="text-separator">|</span> <a href="Modeling.html">Modeling</a> <span class="text-separator">|</span> <a href="Notebook.html">Notebook</a><span class="text-separator">|</span> <a href="Safty.html">Safty</a>
+
-
</div>
+
-
 
+
-
<div class="right">
+
-
<a href="#">Top ^</a>
+
-
</div>
+
-
+
-
<div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
<div id="bottom">
+
-
 
+
-
<div class="center-wrapper">
+
-
  <div class="clearer">&nbsp;</div>
+
-
 
+
-
</div>
+
-
 
+
-
</div>
+
-
 
+
-
</body>
+
-
</html>
+

Latest revision as of 02:37, 27 September 2012

Untitled Document

  • Experiment

Oil pollution is an increasingly grave environmental problem. Artificial hydrocarbonoclastic bacterium plays a critical role in the bioremediation of oil polluted marine systems.
Since this problem has long been tricky and not solved yet, we plan to construct a complex system to degrade oil spill in Escherichia coil, which mainly consists of 3 circuits: degradation circuit, emulsification circuit and stress tolerance circuit. Moreover, there are some other assistant circuits, RBS optimizing and subsequent dehydrogenation for example.
More details please see the Project
In the degradatiom circuit, we studyed two gene involes in it, P450, a cytochrome, which responsible for the medium-chain-length chain and long chain hydrocarbone. AlkB2, a Terminal Oxidase, which oxidate the hydroxy to carboxyl.
In the emulsification circuit, one gene OPRF which encode one enzyme, that can produce the glycolipid for the emucification of the oil spill.
In the sensing circuit, we found to two gene, Alks, which encodes one transcriptional factor, can sense the existence of hydrocarbon, then bind directly to the induction promoter, and the Gnt, also one transcriptional factor, can sense the level of AMP, in our project, we want to have one circuits that can sense the low energy state from saccharides, and turn on the state of using hydrocabone.
Our plane just change the forepart of the normal glucose degration sysyterm, use an hydrocarbone degradation instead, but the dowstream proceed are the same.

  • Sponsors:

right bottom left bottom

Retrieved from "http://2012.igem.org/Team:CD-SCU-CHINA"