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Team:Caltech/Notebook/material/Biofuel Protocols
From 2012.igem.org
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| + | <h5>Ethanol Color Assay Reaction</h4> | ||
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Revision as of 22:39, 2 July 2012
Biofuel Protocols
Ethanol Assay
Ethanol Color Assay Reaction
Materials
- 100 uL ethanol
- 5 mL water
Method
The ethanol assay uses the following reagants and consists of the reaction depicted below. MTT Formazan appears purple, and it is due to this color change that the assay works.
- Make a serial dilution of ethanol. As shown below, we found a linear concentration to absorbance relation for concentrations under .05% ethanol.
Back to Materials and Methods
