Team:Korea U Seoul/Human Practice/Sharing

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         <h4><p id="title">Sharing</p></h4>
         <h4><p id="title">Sharing</p></h4>
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     <dt>
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<b> A. Participation in Creative Challenger Program </b>
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<b> A. Participation in Creative Challenger Program </b>
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</dt>
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</dt>
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            <br>
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        <dd>
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            <dd>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Creative Challenger Program (CCP) is a Korea University program that is dedicated to promote creativity in undergraduates by letting them form groups and engage in self-designed projects, while providing them with a suitable environment. It is sponsored by Center for Teaching and Learning (CTL), and they carefully observe, judge teams and their projects, while providing support. Topics for projects include natural sciences, liberal arts/society, culture/art/sports, economics/management, and engineering/information technology. Despite the wide range of topics, teams are encouraged to associate, making this program a center for idea sharing. Korea U Seoul has participated in CCP since 2010, and through this program, we have been able to spread information about iGEM and Synthetic Biology. In January and September of every year, all the teams and each teams' advisors gather together and have a question and answer session. Doing so, we could share our topics and advance it further. Job-Ara team which participates in CCP has asked us if we could provide an orientation about our project and a lab tour for their students (for primary, middle, highschool students) job search program. We are reviewing the offer currently. What we have now to look forward to is the January final presentation and poster presentation. Korea U Seoul team will continue to perform research for better results even after iGEM is over.  
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                <br>
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</p>
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                <p>
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</dd>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Creative Challenger Program (CCP) is a Korea University program that is dedicated to promote creativity in undergraduates by letting them form groups and engage in self-designed projects, while providing them with a suitable environment. It is sponsored by Center for Teaching and Learning (CTL), and they carefully observe, judge teams and their projects, while providing support. Topics for projects include natural sciences, liberal arts/society, culture/art/sports, economics/management, and engineering/information technology. Despite the wide range of topics, teams are encouraged to associate, making this program a center for idea sharing.KoreaUSeoul has participated in CCP since 2010, and through this program, we have been able to spread information about iGEM and Synthetic Biology. In January and September of every year, all the teams and each teams' advisors gather together and have a question and answer session. Doing so, we could share our topics and advance it further. Job-Ara team which participates in CCP has asked us if we could provide an orientation about our project and a lab tour for their students (for primary, middle, highschool students) job search program. We are reviewing the offer currently.What we have now to look forward to is the January final presentation and poster presentation. KoreaUSeoul team will continue to perform research for better results even after iGEM is over.  
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</p>
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        <dt>
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                <p>
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        <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sulfur plays an important role in activity of AX21; it won’t work without sulfur
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<b> B. Informing iGEM to Students </b>
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</p>
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</dt>
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                <p>
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        <dd>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;overexpression of raxST(sulfur attachment to AX21), RaxR/Q(transformation of PAPS into ATP) is possible in E.coli
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        <br>
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</p>               
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        <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/KUS_iGEM_present.png" width="500" align="center" /></div>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;On August 13, our team member Haerim introduced iGEM to the students who participated in the Korea University Science camp for middle school young scientists. She prepared presentation and explained what synthetic biology is and what we do in iGEM competition. The students showed curiosity and gave lots of questions. They were surprised to know that there is an international jamboree in which people all around the world get together, having a festival to share their academic interests.
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                <b>&nbsp;&nbsp;Advisor’s advice</b>
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</p>
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                <p>
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</dd>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  test any promoter which binds to raxR/H
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</p>
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        <dt>
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                <p>
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        <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  is it possible for Xanthomonas to produce AX21 without plant receptor?
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<b> C. Presentation Contest </b>
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</p>
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</dt>
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                <p>
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        <dd>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  (Professor’s comment) we can run a test using E/coli which has plant receptor
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        <br>
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</p>
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        <div align="center"><img src="https://static.igem.org/mediawiki/2012/d/d1/KUS_presentation_contest.png" width="500"/></div>
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                <p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;During February, Sanghoon, Haerim and Jinsik(one of the 2011 iGEM Korea U Seoulteam members) participated in a presentation competition held by Seoul National University. We made a presentation on synthetic biology, reasoning why we have to invest in this field and reporting the current state of Korea. We mentioned iGEM competition showing that there are increasing interests in synthetic biology area. We made a good result and advanced to the finals.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Method for detecting AX21 production?
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</p>
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</p>
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</dd>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  (Professor’s comment) use mass spectrometry or put florescence after ax21 gene then cut with restriction enzyme
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        <dt>
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</p>
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        <br>
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<b> D. British Columbia UniversitySurvey </b>
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                <b>&nbsp;&nbsp;Jihoon Jung </b>
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</dt>
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                <p>
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        <dd>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Find A
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Every member of Korea U Seoul actively participated in survey held by British Columbia University. The survey was about patent.
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</p>
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</p>
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</dd>
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                <b>&nbsp;&nbsp;Kyeongwoo Jang’s opinion </b>
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                <p>
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        <dt>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;find system which utilizes logic gate in natural world
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        <br>
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</p>
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<b> E. Highschool Observer </b>
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</dt>
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                <br>
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        <dd>
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<p><br>&nbsp;&nbsp;&nbsp;
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                <b> 2) Next meeting </b>
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< A letter from Byunguk Kang(the observer student) ><br><br>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(1) research rax operon
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</p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;How to detect Ax21 : HyunKee Kim
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Research raxR/H paper : Byeongnam Min
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Research Xanthomonas : Jeongmin
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Activity of the substance only with raxR/H:  Haerim Song
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(2) team
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Logic gate: Jihoon Jung, Kyeongwoo Jang
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Science camp presentation:  Haerim Song
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Wiki update: Byeongnam Min, Kyeongwoo Jang, Sanghoon Han
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Notebook translation: HyunKee Kim
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Study Modeling: Sanghoon Han, Byeongnam Min
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song
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                </p>
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            <br><br><br>
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            </dd>
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                <dt>
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<b> B. August 8, 2012 </b>
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</dt>
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            <p style="padding-left:30px;">We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit</p>
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            <br>  
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            <dd>
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<b> 1) Idea development </b>           
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  raxR, raxH can be detected using His tag; however, experiments using cloning technique don’t use this.
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  No indication of functional opetation only with raxR, raxH
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                </p>
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                <b>&nbsp;&nbsp;Haerim Song</b>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Ever since I went to a science camp and met KAIST iGEM team there, I had a great interest in the field of synthetic biology, and registered for 'high school iGEM competition' this year. However, because our team was not able to get support from other laboratories nearby that we asked, our team could not complete our project and could not participate in the jamboree. I kept on researching, and finally sent an e-mail to professor Choi of Computational and Synthetic Biology Lab at Korea University, to have some research experiences. Thankfully, professor Choi and the faculty members of the laboratory provided me a great opportunity to serve as an intern during summer vacation to learn all the basic protocols and experiments on synthetic biology. While I received extensive training, professor Choi allowed me to join the "Korea U Seoul" iGEM team, as a high school observer. I summarized the basic project idea and presented at the lab meeting, and partly involved in PCR and assembly procedure of the project. Through such unique and beneficial experience, I can now design a workable device, (currently conducting personal biosensor research as first author) and get the big picture of how engineers and scientists realize their ideas. I am also willing to participate collegiate iGEM competition next year when I go tocollege. I thank everyone who has given me this great opportunity. 
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                <p>
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</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  raxH without transmembrane segment was autophosphorylation, but no raxR transphosphrylation.
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</dd>
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                </p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  raxH H222 and RaxR D51 is responsible for phosphorylation.
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                </p>
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  <br><br>
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  <div id="wrapperB" style="width:603px; height:63px; ">
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                <b>&nbsp;&nbsp;Lab team</b>
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                 <a href="http://korea.ac.kr">  
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                <p>
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<img src="https://static.igem.org/mediawiki/2012/a/ab/KUS_Ku.jpg" style="padding-top:10px;" width="80px" ></a>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  acquisition of PCEL, raxRH promoter, mRFP using PCR
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                </p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  cloning is expected to be held this week
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                </p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  raxR/H is constitutive, so use other promoter
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                </p>
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                <br>
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                <b> 2) Team placement and H.W </b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;logic gate: find A: Sanghoon Han, Jihoon Jung, Kyeongwoo Jang
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</p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Ax21 production: Wonwook Lee, JungMin, Kyeongjin, Haerim Song, Byeongnam Min, Hyunkee Kim
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Brochure: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam,  Haerim Song
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Science camp presentation:  Haerim Song
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Wiki update: Nam, Byeongnam Min, Kyeongwoo Jang, Sanghoon Han
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Notebook translation: HyunKee Kim
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Study Modeling: Sanghoon Han, Byeongnam Min
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Design team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;CCP: Sanghoon Han
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;KAIST Contact; Byeongnam Min
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Team attire: Jihoon Jung
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;upload at least one copy of design to the homepage (mandatory)
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                </p>
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            </dd>
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            <br><br><br>
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            <dt>
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<b> C. August 15, 2012 </b>
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</dt>
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            <br>
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            <p style="padding-left:30px;">We have discussed about how to dectect and kill 'Xanthomonas oryzae.’ Solidifying the idea and circuit</p>
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            <dd>
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<b> Idea development </b>
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                 <br>
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                <b>&nbsp;&nbsp;Kyeongwoo Jang: logic gate biosensing</b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  researched logic gate used by organism to detect certain chemical
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  British Columbia University already did similar project; it was about concentration dependent color changing bacteria.
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                </p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Logic gate using single step is more efficient than using several input and output steps
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                </p>
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                <b>&nbsp;&nbsp;Sanghoon Han: finding ‘a’ chemical</b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  DSF(diffusible signaling factor)
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</p>
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                <b>&nbsp;&nbsp;Haerim Song: bacterial leaf blight</b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  had presentation about iGEM during ‘Lifescience camp’
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  in order to prevent bacterial leaf blight, chemical-pesticide was given. However, it was proven to be ineffective.  
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Bacterial leaf blight resistant plant was developed but, later sub-population eliminated effect of ‘gene R.’
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</p>
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                <b>&nbsp;&nbsp;Wonwook Lee: sponsor </b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  LG lifescience, Celltrion, Rural development administration, cosmogenetech: yet positive reply, he will continue to contact.
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</p>
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                <b>&nbsp;&nbsp;Sanghoon Han </b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Small Protein-Mediated Quorum Sensing in a Gram- Negative Bacterium (PMID : 22174954) To elucidate this function, we previously isolated and characterized eight genes required for Ax21 activity (rax genes). raxA, raxB and raxC encode components of a predicted type I secretion system (TOSS). Ax21requires this RaxABC TOSS for activity and secretion [3,4]. The RaxB protein carries two highly conserved N terminal proteolytic subdomains characteristic of transporters in Gram-positive bacteria that cleave N-terminal peptides prior to substrate secretion [4]. These data, together with the presence of a predicted N-terminal signal sequence in Ax21, suggest that Ax21 is cleaved by the RaxB transporter prior to secretion. raxST, raxP and raxQ encode enzymes involved in sulfation
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  This indicates AX21 is synthesized without a plant
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</p>        
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                 <br>
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                 <a href="http://compbio.korea.ac.kr/wiki/index.php/Main_Page"> <img src="https://static.igem.org/mediawiki/2012/5/5d/KUS_Csbl.jpg" width="82px" ></a>
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                <b> 2) Design </b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Benchmark : http://www.linocoms.co.kr/index.htm
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</p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;need to use ‘Flash’ program
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                </p>
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                <br>
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                <b> 3) CCP: report due on 24th (logic gate and DIY) </b>
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            </dd>
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<br><br><br>
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                <a href="http://ctl.korea.ac.kr/index.ctl"> <img src="https://static.igem.org/mediawiki/2012/4/49/KUS_Ctl.jpg" width="75px" ></a>
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            <dt>
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<b> D. August 22, 2012 </b>
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</dt>
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            <p style="padding-left:30px;">We discussed about our wiki page design, content, uniform design, and part allocation.</p>
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            <br>
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            <dd>
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<b> 1) iGEM participation fee </b>
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                <br>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Regional Jamboree registration : 275 USD
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</p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Airfare : 40~50 KRW
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                </p>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  Hotel reservation : iGEM will propose several hotels in a discount charge
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                </p>        
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                 <br>
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                 <a href="http://www.cosmogenetech.com/ko">  
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<img src="https://static.igem.org/mediawiki/2012/8/81/KUS_Cosmo.jpg" width="75px" height="70"></a>
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                <b> 2) assigned part </b>
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                <p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sanghoon Han : CCP report, video making, researching logic gate background & design, modeling, Note-book, protocol production
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                <a href="http://www.youtube.com/watch?v=vr7TlPjyAEg">  
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</p>
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<img src="https://static.igem.org/mediawiki/2012/c/c4/KUS_Youtube.jpg" width="65px" ></a>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Jihoon Jung : CCP report, video making, uniform order(later)
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                </p>
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                 <a href="http://www.facebook.com/2012iGEM.KU"> <img src="https://static.igem.org/mediawiki/2012/7/71/KUS_Facebook.jpg" width="52px"></a>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Kyeongwoo Jang: CCP report , video making, logic gate background & design
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                </p>
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                 <a href="https://twitter.com/iGEM_KU2012"> <img src="https://static.igem.org/mediawiki/2012/4/4d/KUS_Twitter.jpg" width="63px"></a>  
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                 <p>
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      </div>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Jeongmin Lee: Xanthomonas Abstract & background
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                </p>
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  <br><br> </div>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Hyunkee Kim : Xanthomonas Abstract & background, Note-book, Safety question
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Haerim Song: mainframe design, poster design(later)
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                </p>
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                 <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Byeongnam Min: modeling
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Jihyong Nam: protocol
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Kyeongjin Kim: get sponsor
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                </p>
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                <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Wonwook Lee : get sponsor
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                </p>
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-
               
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                <br>
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                <b> 3) Please update your info in English by next Wednesday </b>
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            </dd>
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    </div>
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   </div>
   </div>
</div>
</div>

Latest revision as of 02:18, 27 September 2012

Sharing

A. Participation in Creative Challenger Program

     Creative Challenger Program (CCP) is a Korea University program that is dedicated to promote creativity in undergraduates by letting them form groups and engage in self-designed projects, while providing them with a suitable environment. It is sponsored by Center for Teaching and Learning (CTL), and they carefully observe, judge teams and their projects, while providing support. Topics for projects include natural sciences, liberal arts/society, culture/art/sports, economics/management, and engineering/information technology. Despite the wide range of topics, teams are encouraged to associate, making this program a center for idea sharing. Korea U Seoul has participated in CCP since 2010, and through this program, we have been able to spread information about iGEM and Synthetic Biology. In January and September of every year, all the teams and each teams' advisors gather together and have a question and answer session. Doing so, we could share our topics and advance it further. Job-Ara team which participates in CCP has asked us if we could provide an orientation about our project and a lab tour for their students (for primary, middle, highschool students) job search program. We are reviewing the offer currently. What we have now to look forward to is the January final presentation and poster presentation. Korea U Seoul team will continue to perform research for better results even after iGEM is over.


B. Informing iGEM to Students

     On August 13, our team member Haerim introduced iGEM to the students who participated in the Korea University Science camp for middle school young scientists. She prepared presentation and explained what synthetic biology is and what we do in iGEM competition. The students showed curiosity and gave lots of questions. They were surprised to know that there is an international jamboree in which people all around the world get together, having a festival to share their academic interests.


C. Presentation Contest

     During February, Sanghoon, Haerim and Jinsik(one of the 2011 iGEM Korea U Seoulteam members) participated in a presentation competition held by Seoul National University. We made a presentation on synthetic biology, reasoning why we have to invest in this field and reporting the current state of Korea. We mentioned iGEM competition showing that there are increasing interests in synthetic biology area. We made a good result and advanced to the finals.


D. British Columbia UniversitySurvey

     Every member of Korea U Seoul actively participated in survey held by British Columbia University. The survey was about patent.


E. Highschool Observer


    < A letter from Byunguk Kang(the observer student) >

     Ever since I went to a science camp and met KAIST iGEM team there, I had a great interest in the field of synthetic biology, and registered for 'high school iGEM competition' this year. However, because our team was not able to get support from other laboratories nearby that we asked, our team could not complete our project and could not participate in the jamboree. I kept on researching, and finally sent an e-mail to professor Choi of Computational and Synthetic Biology Lab at Korea University, to have some research experiences. Thankfully, professor Choi and the faculty members of the laboratory provided me a great opportunity to serve as an intern during summer vacation to learn all the basic protocols and experiments on synthetic biology. While I received extensive training, professor Choi allowed me to join the "Korea U Seoul" iGEM team, as a high school observer. I summarized the basic project idea and presented at the lab meeting, and partly involved in PCR and assembly procedure of the project. Through such unique and beneficial experience, I can now design a workable device, (currently conducting personal biosensor research as first author) and get the big picture of how engineers and scientists realize their ideas. I am also willing to participate collegiate iGEM competition next year when I go tocollege. I thank everyone who has given me this great opportunity.





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