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Team:SDU-Denmark/labwork/Notebook/week9
From 2012.igem.org
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| - | After 48 hours in the incubator, the 1-FFT colonies finaly reached a high enough concentration for sequencing. | + | After 48 hours in the incubator, the 1-FFT colonies finaly reached a high enough concentration for sequencing.<br/><br/> |
| - | Meanwhile, all the SST colonies had died out. To avoid unnecesary excess work, we simply ran PCR with all mutagenesis primers on an older 1-SST containing | + | Meanwhile, all the SST colonies had died out. To avoid unnecesary excess work, we simply ran PCR with all mutagenesis primers on an older 1-SST containing pJET plasmid. Miraculous, both gel band length and check digest on the resulting sequence yielded perfect results. Why had we not done this before?<br/><br/> |
| - | pJET plasmid. Miraculous, both gel band length and check digest on the resulting sequence yielded perfect results. Why had we not done this before? | + | |
| - | In the following days 1-SST ligated into pJET, grown on ampicillin plates, transformed into Top10, transfered to liquid medium and miniprepped for sequencing. | + | In the following days 1-SST ligated into pJET, grown on ampicillin plates, transformed into Top10, transfered to liquid medium and miniprepped for sequencing.<br/> |
</p> | </p> | ||
Latest revision as of 02:15, 27 September 2012
Laboratory Notebook
27-08-2012 to 02-09-2012
After 48 hours in the incubator, the 1-FFT colonies finaly reached a high enough concentration for sequencing.
Meanwhile, all the SST colonies had died out. To avoid unnecesary excess work, we simply ran PCR with all mutagenesis primers on an older 1-SST containing pJET plasmid. Miraculous, both gel band length and check digest on the resulting sequence yielded perfect results. Why had we not done this before?
In the following days 1-SST ligated into pJET, grown on ampicillin plates, transformed into Top10, transfered to liquid medium and miniprepped for sequencing.
