Team:LMU-Munich/Lab Notebook/Protocols

From 2012.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo10.jpg}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo10.jpg}}
-
[[File:Protocols_banner.jpg|620px|link=]]
+
[[File:Protocols banner.resized WORDS.JPG|620px|link=]]
-
 
-
==Protocols==
 
On this page, we offer our unique protocols to the public.
On this page, we offer our unique protocols to the public.
Line 52: Line 50:
PCR protocol for long flanking homology PCR. You might need those PCR products for interrupting genes with resistance cassettes.
PCR protocol for long flanking homology PCR. You might need those PCR products for interrupting genes with resistance cassettes.
 +
 +
[https://static.igem.org/mediawiki/2012/6/67/LMU-Munich_2012_Removal_of_germination_genes.pdf Example: Using LFH PCR to replace germination genes with resistance cassettes]
 +
 +
An explanation of how we used this protocol for how we knocked out our germination genes using LFH.
  </div>
  </div>
Line 67: Line 69:
<div class="box">
<div class="box">
-
9. Mikroskopie der Sporen
+
9. [https://static.igem.org/mediawiki/2012/e/e9/LMU-Munich_2012_Protocol_for_enhancement_of_mature_spore_numbers.pdf Enhancement of mature spore numbers]
 +
 
 +
Protocol to enrich the spore numbers and purification from vegetative cells.
</div>
</div>
Line 77: Line 81:
<div class="box">
<div class="box">
-
11. Plate reader assay (can also be used with E. coli cells)
+
11. [https://static.igem.org/mediawiki/2012/e/e6/LMU-Munich_2012_Protocol_Plate_Reader.pdf Luminescence measurement]
 +
 
 +
This protocol explains how cultures were treated in advance of a plate reader assay.
</div>
</div>
Line 87: Line 93:
<div class="box">
<div class="box">
-
E. coli antibiotics concentrations
+
[http://partsregistry.org/Help:Protocols/Competent_Cells Competent Cells]
 +
 
 +
''E.coli'' competent cells and transformation. We used the [http://ecoliwiki.net/colipedia/index.php/XL-1_Blue XL1 blue strain].
</div>
</div>
<div class="box">
<div class="box">
-
E.coli trafo
+
[https://static.igem.org/mediawiki/2012/1/1f/LMU-Munich_2012_Alkaline_Lysis_Plasmid_Preparation.pdf Alkaline Lysis Plasmid Preparation]
-
</div>
+
-
<div class="box">
+
Alkaline Lysis Plasmid Preparation for ''E. coli''
-
E.coli dreck-prep
+
</div>
</div>
<div class="box">
<div class="box">
-
Ligation, Verdau
+
[http://openwetware.org/wiki/Silver:_Restriction_Digest Restriction digest]
-
</div>
+
 
 +
[http://openwetware.org/wiki/Silver:_Ligation Ligation]
-
<div class="box">
+
Standard restriction digest and ligation. We often used longer incubation times (up to over night) and did not dephosphorylate the backbones, if they had incompatible sticky ends.
-
Kompetente E.colis
+
</div>
</div>
<div class="box">
<div class="box">
-
lacZ assay in E.coli
+
[https://static.igem.org/mediawiki/2012/b/bb/LMU-Munich_2012_%C3%9F-Galactosidase_Assay_E._coli.pdf β-Galactosidase Assay in ''E. coli'']
-
</div>
+
-
<div class="box">
+
Quantitative ''lacZ''-assay in ''E.coli''.
-
(Proteinase K)
+
</div>
</div>
-
<div class="box">
+
 
-
(Western Blot)
+
-
</div>
+
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 02:00, 27 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo10.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde