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Team:Tokyo-NoKoGen/Achievement
From 2012.igem.org
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<BR>Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator</b> | <BR>Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator</b> | ||
<BR> | <BR> | ||
| - | <BR>Tokyo-NoKoGen 2010 created a chimeric sensory rhodopsin with a chemotaxis transducer tar from <i>E. coli</i> (BBa_K225001). We created a chimeric sensor rhodopsin, the same sensor domain of rodopsin from <i>Natronobacterium pharaonis (N. pharaonis)</i>, and tested to see if the GFP under PompC will show any expression under the light, the light signal received by the sensory rhodopsin. The figure below shows the result. As you can see, we have found that | + | <BR>Tokyo-NoKoGen 2010 created a chimeric sensory rhodopsin with a chemotaxis transducer tar from <i>E. coli</i> (BBa_K225001). We created a blue light sensor, a chimeric sensor rhodopsin, the same sensor domain of rodopsin from <i>Natronobacterium pharaonis (N. pharaonis)</i>, and tested to see if the GFP under PompC will show any expression under the light, the light signal received by the sensory rhodopsin. The figure below shows the result. As you can see, we have found that |
| + | opposing to what we expected, the blue light sensor responded to light by turning off the expression of GFP, and turns on GFP expression in the dark. | ||
<BR> | <BR> | ||
<div align=center><img src=https://static.igem.org/mediawiki/2012/thumb/1/16/Rhodopsinfig5.png/711px-Rhodopsinfig5.png height=60% width=60%></div> | <div align=center><img src=https://static.igem.org/mediawiki/2012/thumb/1/16/Rhodopsinfig5.png/711px-Rhodopsinfig5.png height=60% width=60%></div> | ||
Revision as of 01:38, 27 September 2012
Tokyo-NoKoGen
2012
- achievement
Achievement
We have successfully managed to accomplish the following task shown in the table below (Fig.1)
Fig.1 Table of a list of our achievement
We have also managed to successfully construct and evaluate the following BioBrick
Sensory rhodopsin
BBa_K769003
Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator
Tokyo-NoKoGen 2010 created a chimeric sensory rhodopsin with a chemotaxis transducer tar from E. coli (BBa_K225001). We created a blue light sensor, a chimeric sensor rhodopsin, the same sensor domain of rodopsin from Natronobacterium pharaonis (N. pharaonis), and tested to see if the GFP under PompC will show any expression under the light, the light signal received by the sensory rhodopsin. The figure below shows the result. As you can see, we have found that opposing to what we expected, the blue light sensor responded to light by turning off the expression of GFP, and turns on GFP expression in the dark.
The lux operon
Left:BBa_K769022
Middle:BBa_K769011
Right:BBa_K769020
BBa_K769011
Promoter(Pbad)-RBS-lux operon-DT
BBa_K769020
lux operon(K769011) + lumP(K769019)
BBa_K769022
lux operon(K769011) + GFP(I13522)
