"
Team:Paris-Saclay/Project/Notebook/Week 11
From 2012.igem.org
(Difference between revisions)
YohannPetiot (Talk | contribs) |
(→17th august) |
||
| (12 intermediate revisions not shown) | |||
| Line 23: | Line 23: | ||
</head> | </head> | ||
<body> | <body> | ||
| - | <div id="single- | + | <div id="tiles"> |
| - | <div | + | <div id="single-tile3" class="red live-tile" data-mode="flip" data-delay="4000"> |
| - | + | <div> | |
| - | + | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> | |
| - | + | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | |
| + | <img src="https://static.igem.org/mediawiki/2012/7/7c/Project-1.png" alt="" /> | ||
| + | </a> | ||
| + | </div> | ||
<div> | <div> | ||
| - | <img src=" | + | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> |
| - | + | <img src="https://static.igem.org/mediawiki/2012/d/d1/Project2.jpg" alt="" /> | |
| + | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
| + | </a> | ||
</div> | </div> | ||
| - | <script type="text/javascript"> | + | </div> |
| + | <script type="text/javascript"> | ||
// apply regular slide universally unless .exclude class is applied | // apply regular slide universally unless .exclude class is applied | ||
// NOTE: The default options for each liveTile are being pulled from the 'data-' attributes | // NOTE: The default options for each liveTile are being pulled from the 'data-' attributes | ||
$(".live-tile, .flip-list").not(".exclude").liveTile(); | $(".live-tile, .flip-list").not(".exclude").liveTile(); | ||
| - | </script> | + | </script> |
| - | </div> | + | </div> |
</body> | </body> | ||
</html> | </html> | ||
| Line 43: | Line 49: | ||
</div> | </div> | ||
<div id="content-paris-saclay"> | <div id="content-paris-saclay"> | ||
| - | + | ='''Week 11'''= | |
| - | [[Category:Team:Paris-Saclay/Project Gemote/Notebook| | + | |
| + | |||
| + | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|k]] | ||
__NOTOC__ | __NOTOC__ | ||
====13th August==== | ====13th August==== | ||
| Line 65: | Line 73: | ||
{| width="85%" | {| width="85%" | ||
|- | |- | ||
| - | | style="width: 50%;"| | + | | style="width: 50%;"|Determination of the concentration of plasmid pSB1A2 which has been linearized by HINDIII. We are expecting a band at size (2079+935) ~3kbp |
| style="width: 35%;"| [[File:Week11-4.jpg|right|280px]] | | style="width: 35%;"| [[File:Week11-4.jpg|right|280px]] | ||
|- | |- | ||
| - | | style="width: 50%;"|A new PCR of the plasmid pSB1A2 is realized to obtain more quantity of the plasmid. We are expecting a band at | + | | style="width: 50%;"|A new PCR of the plasmid pSB1A2 is realized to obtain more quantity of the plasmid. We are expecting a band at size 2079 bp. |
| style="width: 35%;"| [[File:Week11-5.jpg|right|280px]] | | style="width: 35%;"| [[File:Week11-5.jpg|right|280px]] | ||
|} | |} | ||
| Line 74: | Line 82: | ||
PCR program used: | PCR program used: | ||
[[File:Week11-6.jpg|500px]] | [[File:Week11-6.jpg|500px]] | ||
| - | |||
====14th August==== | ====14th August==== | ||
| Line 80: | Line 87: | ||
{| width="85%" | {| width="85%" | ||
|- | |- | ||
| - | | style="width: 50%;"| | + | | style="width: 50%;"|Determination of the concentration of the BBa_K274100 by electrophoresis on a gel at O.8% Agarose. We are expecting a band at size 2100bp |
| style="width: 35%;"| [[File:Week11-7.jpg|right|280px]] | | style="width: 35%;"| [[File:Week11-7.jpg|right|280px]] | ||
|} | |} | ||
| Line 88: | Line 95: | ||
**PSB1A3Amil CP + Ampicilline | **PSB1A3Amil CP + Ampicilline | ||
**PSB1C3 Amil GFP + Chloramphinicol | **PSB1C3 Amil GFP + Chloramphinicol | ||
| - | |||
| - | |||
====15th August==== | ====15th August==== | ||
| - | + | French holiday. | |
| - | + | ||
====16th August==== | ====16th August==== | ||
| Line 113: | Line 117: | ||
{| width="85%" | {| width="85%" | ||
|- | |- | ||
| - | | style="width: 50%;"|Visualization of the DPNI’s digestion of the plasmid pSB1A2 by 0.8% Agarose gel electrophoresis. . We are expecting a band at | + | | style="width: 50%;"|Visualization of the DPNI’s digestion of the plasmid pSB1A2 by 0.8% Agarose gel electrophoresis. . We are expecting a band at size 2079 bp. |
| style="width: 35%;"| [[File:Week11-10.jpg|right|220px]] | | style="width: 35%;"| [[File:Week11-10.jpg|right|220px]] | ||
|} | |} | ||
| - | *Miniprep of the | + | *Miniprep of the pSB1A2 plasmid. |
*Miniprep of BBa_K274100 and BBa_K115017 | *Miniprep of BBa_K274100 and BBa_K115017 | ||
*Gibson assembly of the B construction | *Gibson assembly of the B construction | ||
| Line 123: | Line 127: | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
{{Team:Paris-Saclay/Footer}} | {{Team:Paris-Saclay/Footer}} | ||
Latest revision as of 00:39, 27 September 2012
Week 11
13th August
| Purification by PCR clean-up of BBa_K115017 treated with DPNI. To visualize the fragment, an electrophoresis has been made with a 2% Agarose gel. We are expecting a band at the size of 123 bp. |  |
 | New PCR of BBa_K115017 to obtain more quantity of the fragment |
PCR program used:
- Digestion by DPNI of the BBa_K115017 in order to degrade the matrix plasmid.
| Determination of the concentration of plasmid pSB1A2 which has been linearized by HINDIII. We are expecting a band at size (2079+935) ~3kbp |  |
| A new PCR of the plasmid pSB1A2 is realized to obtain more quantity of the plasmid. We are expecting a band at size 2079 bp. |  |
PCR program used:
14th August
| Determination of the concentration of the BBa_K274100 by electrophoresis on a gel at O.8% Agarose. We are expecting a band at size 2100bp |  |
- Digestion of the BBa_K274100 by DPNI to degrade the matrix plasmid.
- Stocking up cells with glycerol
- PSB1A3Amil CP + Ampicilline
- PSB1C3 Amil GFP + Chloramphinicol
15th August
French holiday.
16th August
| PCR of BBa_K274100 and visualization by 0.8% Agarose gel electrophoresis. We are expecting a band at 3400bp. |  |
PCR program used:
- Digestion of the BBa_K274100 by DPNI to degrade the matrix plasmid.
17th august
| Visualization of the DPNI’s digestion of the plasmid pSB1A2 by 0.8% Agarose gel electrophoresis. . We are expecting a band at size 2079 bp. |  |
- Miniprep of the pSB1A2 plasmid.
- Miniprep of BBa_K274100 and BBa_K115017
- Gibson assembly of the B construction
- Transformation of DH5α competent cells with the Gibson B construction. The Petri dishes are placed at 37°C.
Follow us !