Team:Paris-Saclay/Project/Notebook/Week 9
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<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> | ||
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
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<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
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- | + | ='''Week 9'''= | |
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|i]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|i]] | ||
__NOTOC__ | __NOTOC__ | ||
+ | ====30th july==== | ||
+ | |||
+ | *Analysis of the Petri dishes after the transformation with the B construction | ||
+ | |||
+ | {| width="85%" | ||
+ | |- | ||
+ | | style="width: 50%;"|Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel. | ||
+ | | style="width: 35%;"| [[File:Week9-1.jpg|right|300px]] | ||
+ | |} | ||
+ | |||
+ | |||
+ | ====31th July==== | ||
+ | |||
+ | *Miniprep of the colonies B1 and B2 that came from the transformation with the B construction. | ||
+ | *Nanovue to measure the concentration of the samples B1, B2 and the control | ||
+ | |||
+ | |||
+ | [[File:Week9-2.jpg|500px|center]] | ||
+ | |||
+ | *Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction. | ||
+ | |||
+ | [[File:Week9-3.jpg|400px|center]] | ||
+ | |||
+ | PCR program used: | ||
+ | |||
+ | [[File:Week9-4.jpg|700px|center]] | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
+ | </div> | ||
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{{Team:Paris-Saclay/Footer}} | {{Team:Paris-Saclay/Footer}} |
Latest revision as of 00:31, 27 September 2012
Week 9
30th july
- Analysis of the Petri dishes after the transformation with the B construction
Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel. |
31th July
- Miniprep of the colonies B1 and B2 that came from the transformation with the B construction.
- Nanovue to measure the concentration of the samples B1, B2 and the control
- Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction.
PCR program used:
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