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Team:Paris-Saclay/Project/Notebook/Week 7
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<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
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<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
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| - | + | ='''Week 7'''= | |
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | ||
__NOTOC__ | __NOTOC__ | ||
| + | *PCR in large volume to increase the amount of available biobricks. | ||
| + | *Treatment with DPN1 to eliminate plasmids. | ||
| + | *Gel purification to only keep fragments of interest. | ||
| + | *Gibson Assembly method to put together the different biobricks for each construction. | ||
| + | *Transformation with assembly products and staggering on a selective medium. | ||
| + | *Colony PCR to have results before the transformation results. | ||
| + | |||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Latest revision as of 00:30, 27 September 2012
Week 7
- PCR in large volume to increase the amount of available biobricks.
- Treatment with DPN1 to eliminate plasmids.
- Gel purification to only keep fragments of interest.
- Gibson Assembly method to put together the different biobricks for each construction.
- Transformation with assembly products and staggering on a selective medium.
- Colony PCR to have results before the transformation results.
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