"
Team:Paris-Saclay/Project/Notebook/Week 7
From 2012.igem.org
(Difference between revisions)
YohannPetiot (Talk | contribs) (Created page with "<div id="large-single-paris-saclay"> {{Team:Paris-Saclay/Header}} <div id="single-paris-saclay"> {{Team:Paris-Saclay/Menu}} <div id="single-left-column"> <html> <head> <l...") |
(→Week 7) |
||
| (5 intermediate revisions not shown) | |||
| Line 23: | Line 23: | ||
</head> | </head> | ||
<body> | <body> | ||
| - | <div id="single- | + | <div id="tiles"> |
| - | <div | + | <div id="single-tile3" class="red live-tile" data-mode="flip" data-delay="4000"> |
| - | + | <div> | |
| - | + | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> | |
| - | + | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | |
| + | <img src="https://static.igem.org/mediawiki/2012/7/7c/Project-1.png" alt="" /> | ||
| + | </a> | ||
| + | </div> | ||
<div> | <div> | ||
| - | <img src=" | + | <a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract"> |
| - | + | <img src="https://static.igem.org/mediawiki/2012/d/d1/Project2.jpg" alt="" /> | |
| + | <div class="child-tile"><p class="child-tile">GEMOTE Project</p></div> | ||
| + | </a> | ||
</div> | </div> | ||
| - | <script type="text/javascript"> | + | </div> |
| + | <script type="text/javascript"> | ||
// apply regular slide universally unless .exclude class is applied | // apply regular slide universally unless .exclude class is applied | ||
// NOTE: The default options for each liveTile are being pulled from the 'data-' attributes | // NOTE: The default options for each liveTile are being pulled from the 'data-' attributes | ||
$(".live-tile, .flip-list").not(".exclude").liveTile(); | $(".live-tile, .flip-list").not(".exclude").liveTile(); | ||
| - | </script> | + | </script> |
| - | </div> | + | </div> |
</body> | </body> | ||
</html> | </html> | ||
| Line 43: | Line 49: | ||
</div> | </div> | ||
<div id="content-paris-saclay"> | <div id="content-paris-saclay"> | ||
| - | + | ='''Week 7'''= | |
| + | |||
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|g]] | ||
__NOTOC__ | __NOTOC__ | ||
| + | *PCR in large volume to increase the amount of available biobricks. | ||
| + | *Treatment with DPN1 to eliminate plasmids. | ||
| + | *Gel purification to only keep fragments of interest. | ||
| + | *Gibson Assembly method to put together the different biobricks for each construction. | ||
| + | *Transformation with assembly products and staggering on a selective medium. | ||
| + | *Colony PCR to have results before the transformation results. | ||
| + | |||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Latest revision as of 00:30, 27 September 2012
Week 7
- PCR in large volume to increase the amount of available biobricks.
- Treatment with DPN1 to eliminate plasmids.
- Gel purification to only keep fragments of interest.
- Gibson Assembly method to put together the different biobricks for each construction.
- Transformation with assembly products and staggering on a selective medium.
- Colony PCR to have results before the transformation results.
Follow us !