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Team:Paris-Saclay/Project/Notebook/Week 7
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| - | *PCR in large volume to | + | *PCR in large volume to increase the amount of available biobricks. |
| - | *Treatment with DPN1 to eliminate plasmids | + | *Treatment with DPN1 to eliminate plasmids. |
| - | *Gel purification to only keep | + | *Gel purification to only keep fragments of interest. |
| - | *Gibson Assembly method to put together the different biobricks for each construction | + | *Gibson Assembly method to put together the different biobricks for each construction. |
| - | *Transformation with assembly products and staggering on a selective medium | + | *Transformation with assembly products and staggering on a selective medium. |
| - | *Colony PCR to have results before the transformation results | + | *Colony PCR to have results before the transformation results. |
Revision as of 00:29, 27 September 2012
Week 7
- PCR in large volume to increase the amount of available biobricks.
- Treatment with DPN1 to eliminate plasmids.
- Gel purification to only keep fragments of interest.
- Gibson Assembly method to put together the different biobricks for each construction.
- Transformation with assembly products and staggering on a selective medium.
- Colony PCR to have results before the transformation results.
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