Team:Paris-Saclay/Project/Notebook/Week 4

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<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract">
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<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
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<a href="https://2012.igem.org/Team:Paris-Saclay/Project/Abstract">
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<img src="http://www.ecole-adn.fr/uploads/2011/09/Probiotics-300x300.jpg" alt="" />
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<img src="https://static.igem.org/mediawiki/2012/d/d1/Project2.jpg" alt="" />
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
<div class="child-tile"><p class="child-tile">GEMOTE Project</p></div>
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{{Team:Paris-Saclay/Follow}}
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='''Week 4'''=
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]]
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]]
__NOTOC__
__NOTOC__
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*We place an order for the primers
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*We placed an order for the primers.
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*DNA (biobricks) Resuspension using iGEM protocol  
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*DNA (biobricks) Resuspension using iGEM protocol.
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*Transformation of each biobricks by thermal shock to have a large quantity of DNA
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*Thermal shock treatment of each biobrick to have a large quantity of DNA.
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*Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid
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*Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
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*We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep  
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*We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
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*Miniprep to purify each biobrick (ready to use)
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*Miniprep to purify each biobrick (ready to use).
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*Groove for two colonies because they did not grown on the liquid culture  
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*Groove for two colonies because they did not grow on the liquid culture.
{{Team:Paris-Saclay/Follow}}
{{Team:Paris-Saclay/Follow}}
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{{Team:Paris-Saclay/Footer}}
{{Team:Paris-Saclay/Footer}}

Latest revision as of 00:29, 27 September 2012

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Week 4

  • We placed an order for the primers.
  • DNA (biobricks) Resuspension using iGEM protocol.
  • Thermal shock treatment of each biobrick to have a large quantity of DNA.
  • Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
  • We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
  • Miniprep to purify each biobrick (ready to use).
  • Groove for two colonies because they did not grow on the liquid culture.

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