"
Team:Paris-Saclay/Project/Notebook/Week 4
From 2012.igem.org
(Difference between revisions)
YohannPetiot (Talk | contribs) |
(→Week 4) |
||
| Line 53: | Line 53: | ||
[[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|d]] | ||
__NOTOC__ | __NOTOC__ | ||
| - | *We | + | *We placed an order for the primers. |
| - | *DNA (biobricks) Resuspension using iGEM protocol | + | *DNA (biobricks) Resuspension using iGEM protocol. |
| - | * | + | *Thermal shock treatment of each biobrick to have a large quantity of DNA. |
| - | *Staggering of the transformed bacteria on a selective medium to select bacteria which | + | *Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid. |
| - | *We | + | *We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep. |
| - | *Miniprep to purify each biobrick (ready to use) | + | *Miniprep to purify each biobrick (ready to use). |
| - | *Groove for two colonies because they did not | + | *Groove for two colonies because they did not grow on the liquid culture. |
| - | + | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Revision as of 00:24, 27 September 2012
Week 4
- We placed an order for the primers.
- DNA (biobricks) Resuspension using iGEM protocol.
- Thermal shock treatment of each biobrick to have a large quantity of DNA.
- Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
- We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
- Miniprep to purify each biobrick (ready to use).
- Groove for two colonies because they did not grow on the liquid culture.
Follow us !