Team:Evry/Notebook/w14

From 2012.igem.org

(Difference between revisions)
(Replaced content with "{{:Team:Evry/template_notebook}} <html> <h1>Week 14: 10th September - 16th September</h1> </html>")
Line 3: Line 3:
<h1>Week 14: 10th September - 16th September</h1>
<h1>Week 14: 10th September - 16th September</h1>
 +
<p> this week we have worked extensively on a batch of different manipulations including:
 +
<ul>
 +
<li>biobricking promotors in Psb1c3 which are:
 +
            <ul><li>P Elastase</li>
 +
                    <li>Hsp70</li>
 +
                    <li>P Car A</li>
 +
                    <li> FLk1</li>
 +
                    <li>P CMV</li></ul>
 +
this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.</ul>
 +
 +
<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
 +
this included PCR amplification of seperate genes GFP-AID and Tir1 using  then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles</li>
 +
<ul>
 +
 +
 +
   
 +
<li>Preparation of chemical Competent Cells</li>
 +
 +
 +
                     
 +
</html>
</html>

Revision as of 00:15, 27 September 2012

Weeks:

June July August September October November

Week 14: 10th September - 16th September

this week we have worked extensively on a batch of different manipulations including:

  • biobricking promotors in Psb1c3 which are:
    • P Elastase
    • Hsp70
    • P Car A
    • FLk1
    • P CMV
    this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
  • Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+: this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles