Team:Nanjing-China

From 2012.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
-
 
+
<html lang="en">  
-
    <!-- Le styles for debug
+
-
    <link rel="stylesheet/less" type="text/css" href="less/page-index.less">
+
-
    <script src="less/less-1.3.0.min.js" type="text/javascript"></script>-->
+
-
 
+
     <link rel="stylesheet" type="text/css" href="http://note.woku1.com/images/css/style.css">
     <link rel="stylesheet" type="text/css" href="http://note.woku1.com/images/css/style.css">
-
 
     <!-- Le HTML5 shim, for IE6-8 support of HTML5 elements -->
     <!-- Le HTML5 shim, for IE6-8 support of HTML5 elements -->
     <!--[if lt IE 9]>
     <!--[if lt IE 9]>
       <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
       <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
     <![endif]-->
     <![endif]-->
-
 
-
    <!-- Le fav and touch icons -->
 
-
    <link rel="shortcut icon" href="ico/favicon.ico">
 
-
    <link rel="apple-touch-icon-precomposed" sizes="144x144" href="ico/apple-touch-icon-144-precomposed.png">
 
-
    <link rel="apple-touch-icon-precomposed" sizes="114x114" href="ico/apple-touch-icon-114-precomposed.png">
 
-
    <link rel="apple-touch-icon-precomposed" sizes="72x72" href="ico/apple-touch-icon-72-precomposed.png">
 
-
    <link rel="apple-touch-icon-precomposed" href="ico/apple-touch-icon-57-precomposed.png">
 
-
 
     <script type="text/javascript">
     <script type="text/javascript">
Line 34: Line 21:
             <span class="icon-bar"></span>
             <span class="icon-bar"></span>
           </button>
           </button>
-
           <a class="brand" href="./index.html">NanjingChina</a>
+
           <a class="brand" href="https://2012.igem.org/Team:Nanjing-China"></a>
           <div class="nav-collapse collapse">
           <div class="nav-collapse collapse">
             <ul class="nav">
             <ul class="nav">
               <li class="active">
               <li class="active">
-
                 <a class="home-link" href="./index.html">Home</a>
+
                 <a class="home-link" href="https://2012.igem.org/Team:Nanjing-China">Home</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="team-link" href="./scaffolding.html">Team</a>
+
                 <a class="team-link" href="https://2012.igem.org/Team:Nanjing-China/Team">Team</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="profile-link" href="./base-css.html">Profile</a>
+
                 <a class="profile-link" href="https://igem.org/Team.cgi?year=2012&team_name=Nanjing-China">Profile</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="project-link" href="./components.html">Project</a>
+
                 <a class="project-link" href="https://2012.igem.org/Team:Nanjing-China/Project">Project</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="parts-link" href="./javascript.html">Parts</a>
+
                 <a class="parts-link" href="https://2012.igem.org/Team:Nanjing-China/Parts">Parts</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="modeling-link" href="./less.html">Modeling</a>
+
                 <a class="modeling-link" href="https://2012.igem.org/Team:Nanjing-China/Partner">Partner</a>
               </li>
               </li>
-
              <li class="divider-vertical"></li>
 
               <li class="">
               <li class="">
-
                 <a class="notebook-link" href="./download.html">Notebook</a>
+
                 <a class="notebook-link" href="https://2012.igem.org/Team:Nanjing-China/Notebook">Notebook</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="safety-link" href="./examples.html">Safety</a>
+
                 <a class="safety-link" href="https://2012.igem.org/Team:Nanjing-China/Safety">Safety</a>
               </li>
               </li>
               <li class="">
               <li class="">
-
                 <a class="attr-link" href="./examples.html">Attributions</a>
+
                 <a class="attr-link" href="https://2012.igem.org/Team:Nanjing-China/Attributions">Attributions</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="human-link" href="https://2012.igem.org/Team:Nanjing-China/Practise">Practise</a>
               </li>
               </li>
             </ul>
             </ul>
Line 73: Line 62:
     <div class="container">
     <div class="container">
       <div class="row">
       <div class="row">
-
         <div class="span3">
+
         <div class="span12">
-
          <div class="well sidebar-nav">
+
-
            <ul class="nav nav-list">
+
-
              <li class="nav-header">Sidebar</li>
+
-
              <li class="active"><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li class="nav-header">Sidebar</li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li class="nav-header">Sidebar</li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
              <li><a href="#">Link</a></li>
+
-
            </ul>
+
-
          </div>
+
-
        </div><!--Sidebar content-->
+
-
        <div class="span9">
+
           <div class="contain-inner well">
           <div class="contain-inner well">
           <div id="myCarousel" class="carousel slide">
           <div id="myCarousel" class="carousel slide">
             <div class="carousel-inner">
             <div class="carousel-inner">
               <div class="item active">
               <div class="item active">
-
                 <img src="" alt="">
+
                <img src="https://static.igem.org/mediawiki/2012/6/6c/Logo_siGEM2.jpg" alt="">
 +
                <div class="carousel-caption">
 +
                <p>Nanjing-China 2012 is working on small interfering genetically engineered machine.</p>
 +
                </div>
 +
              </div>
 +
              <div class="item">
 +
                 <img src="https://static.igem.org/mediawiki/igem.org/5/58/TEAM-NJ.jpg" alt="">
                 <div class="carousel-caption">
                 <div class="carousel-caption">
-
                  <h4>First Thumbnail label</h4>
+
                   <p>Our team</p>
-
                   <p></p>
+
                 </div>
                 </div>
               </div>
               </div>
               <div class="item">
               <div class="item">
-
                 <img src="http://note.woku1.com/images/img/bootstrap-mdo-sfmoma-02.jpg" alt="">
+
                 <img src="https://static.igem.org/mediawiki/igem.org/f/fd/Grow-nj.jpg" alt="">
                 <div class="carousel-caption">
                 <div class="carousel-caption">
-
                  <h4>Second Thumbnail label</h4>
+
                   <p>Engineered lettuce growing</p>
-
                   <p>.</p>
+
                 </div>
                 </div>
               </div>
               </div>
               <div class="item">
               <div class="item">
-
                 <img src="http://note.woku1.com/images/img/bootstrap-mdo-sfmoma-03.jpg" alt="">
+
                 <img src="https://static.igem.org/mediawiki/igem.org/7/73/SEED-NJCN.jpg" alt="">
                 <div class="carousel-caption">
                 <div class="carousel-caption">
-
                  <h4>Third Thumbnail label</h4>
+
                   <p>Igem LOGO</p>
-
                   <p>.</p>
+
                 </div>
                 </div>
               </div>
               </div>
Line 124: Line 94:
             <a class="right carousel-control" href="#myCarousel" data-slide="next">›</a>
             <a class="right carousel-control" href="#myCarousel" data-slide="next">›</a>
           </div><!--/carousel-->
           </div><!--/carousel-->
-
           <h2>Abstract</h2>
+
           <div class="row">
-
          <p>.</p>
+
          <div class="span7 span-pad">
-
           <p>.</p>
+
          <h2>siGEM at a glance</h2>
-
           <p>.</p>
+
          <p class="MsoNormal"><span lang="EN-US">The essential and expanding roles of
-
        </div>
+
small non coding RNAs are becoming clearer to us. More interestingly, plant
 +
endogenous small RNAs through food intake directly influence gene expression in
 +
animals (especially in liver) , as a recent study showed.&nbsp;&nbsp; </span></p>
 +
<p class="MsoNormal"><span lang="EN-US">We took advantage of this
 +
<span class="word">phenomenon</span>, and made engineered lettuce producing
 +
artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha,
 +
whose high expression in liver can cause insulin resistance, as target. (More...
 +
in project section)</span></p>          </div>
 +
          <div class="span3">
 +
          <img src=" https://static.igem.org/mediawiki/igem.org/c/c0/Blank.gif"/>
 +
          </div>
 +
           </div>
 +
 
 +
          <div class="row">
 +
          <div class="span7 span-pad">
 +
          <h2>Parts at a glance</h2>
 +
          <p class="MsoNormal"><span lang="EN-US">Our team designed three main modules for
 +
our project, si/mi RNA and green plant synthetic biology. They may serve as a
 +
bit of ground work for future teams working on plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">They are RNA generating module, T-DNA
 +
module, and Barcode 2 module. </span></p>
 +
<p class="MsoNormal"><span lang="EN-US">&nbsp;RNA generating module make vegetable a
 +
small RNA producing factory.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">Parts in T-DNA module are used for
 +
constructing customized binary vector for plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">Barcode 2 module is a signature system
 +
for engineered plant.</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">&nbsp;</span></p>
 +
<p class="MsoNormal"><span lang="EN-US">(Read more in Parts and project)</span></p>
 +
 
 +
          </div>
 +
          <div class="span3">
 +
          <img src=" https://static.igem.org/mediawiki/igem.org/c/c0/Blank.gif"/>
 +
          </div>
 +
           </div>
 +
 
 +
          <div class="row">
 +
          <div class="span7 span-pad">
 +
          <h2>Sharing</h2>
 +
          <p class="MsoNormal"><span lang="EN-US">You can simply use overlapping PCR to
 +
switch the sequence of small RNAs in part BBa_K788002 to make your own parts and
 +
plant. (Detail is available in the part registry
 +
<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788002">
 +
BBa_K788002</a>). Also, you can use part<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788010">
 +
BBa_K788010 </a>and<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788005">
 +
BBa_K7880005</a> to make new binary vectors. And you may put the signature on
 +
the plant you work with by adding a barcode 2 part at the end of your system
 +
according to rules
 +
<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788007">
 +
BBa_K880007</a> gave.</span></p>         </div>
 +
          <div class="span3">
 +
          <img src=" https://static.igem.org/mediawiki/igem.org/c/c0/Blank.gif"/>
 +
          </div>
 +
          </div>
 +
         
 +
        </div>
         </div><!--Body content-->
         </div><!--Body content-->
       </div>
       </div>
Line 138: Line 163:
       <div class="wrapper outer">
       <div class="wrapper outer">
         <div class="wrapper inner">
         <div class="wrapper inner">
 +
        <div id="footer">
 +
              <div id="f-poweredbyico">
 +
              <a href="http://www.mediawiki.org/">
 +
              <img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki">
 +
              </a>
 +
              </div>      
 +
              <div id="f-copyrightico">
 +
              <a href="http://creativecommons.org/licenses/by/3.0/">
 +
              <img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31">
 +
              </a>
 +
              </div>    
 +
              <ul id="f-list" class="unstyled link-list">
 +
              <li id="t-recentchanges">
 +
              <a href="/Special:RecentChanges" title="Recent changes">Recent changes</a>
 +
              </li>
 +
              <li id="t-whatlinkshere">
 +
              <a href="/Special:WhatLinksHere/Team:HokkaidoU_Japan" title="List of all wiki pages that link here [j]" accesskey="j">What links here</a>
 +
              </li>
 +
              <li id="t-recentchangeslinked">
 +
              <a href="/Special:RecentChangesLinked/Team:HokkaidoU_Japan" title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a>
 +
              </li>
 +
              <li id="t-upload">
 +
              <a href="/Special:Upload" title="Upload files [u]" accesskey="u">Upload file</a>
 +
              </li>
 +
              <li id="t-specialpages">
 +
              <a href="/Special:SpecialPages" title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
              </li>
 +
              <li><a href="/Special:Preferences">My preferences</a></li>
 +
              </ul>
 +
            </div>
 +
            <ul id="f-list" class="unstyled link-list">
 +
            <li id="t-print" style="padding-left:180px">
 +
            <a href="/wiki/index.php?title=Team:HokkaidoU_Japan&amp;printable=yes" title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 +
            <li id="t-permalink">
 +
            <a href="/wiki/index.php?title=Team:HokkaidoU_Japan&amp;oldid=263009" title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
 +
            <li id="privacy">
 +
            <a href="/2011.igem.org:Privacy_policy" title="2011.igem.org:Privacy policy">Privacy policy</a>
 +
            </li>
 +
            <li id="disclaimer">
 +
            <a href="/2011.igem.org:General_disclaimer" title="2011.igem.org:General disclaimer">Disclaimers</a>
 +
            </li>
 +
        </ul>
 +
        <ul class="unstyled link-list">
 +
        <li><a href="www.nju.edu.cn"><img src="https://static.igem.org/mediawiki/igem.org/4/46/Nju_logo.png" /></a></li>
 +
        <li><a href="igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/igem.org/0/08/Igem_logo.png" /></a></li>
 +
        </ul>
         </div>
         </div>
       </div>
       </div>
Line 150: Line 224:
     window.jQuery || document.write('<script src="js/jquery-1.7.1.min.js"><\/script>')
     window.jQuery || document.write('<script src="js/jquery-1.7.1.min.js"><\/script>')
     </script>
     </script>
 +
    <script src="http://note.woku1.com/images/js/bootstrap-carousel.js" type="text/javascript"></script>
     <!-- Analytics
     <!-- Analytics
     ================================================== -->
     ================================================== -->
     <script>
     <script>
-
      $(document).ready(function(){
+
    $(document).ready(function(){
         $('#roll').hide();
         $('#roll').hide();
         $(window).scroll(function() {
         $(window).scroll(function() {
Line 168: Line 243:
       });
       });
     </script>
     </script>
 +
</html>

Latest revision as of 00:13, 27 September 2012

siGEM at a glance

The essential and expanding roles of small non coding RNAs are becoming clearer to us. More interestingly, plant endogenous small RNAs through food intake directly influence gene expression in animals (especially in liver) , as a recent study showed.  

We took advantage of this phenomenon, and made engineered lettuce producing artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha, whose high expression in liver can cause insulin resistance, as target. (More... in project section)

Parts at a glance

Our team designed three main modules for our project, si/mi RNA and green plant synthetic biology. They may serve as a bit of ground work for future teams working on plant.

They are RNA generating module, T-DNA module, and Barcode 2 module.

 RNA generating module make vegetable a small RNA producing factory.

Parts in T-DNA module are used for constructing customized binary vector for plant.

Barcode 2 module is a signature system for engineered plant.

 

(Read more in Parts and project)

Sharing

You can simply use overlapping PCR to switch the sequence of small RNAs in part BBa_K788002 to make your own parts and plant. (Detail is available in the part registry BBa_K788002). Also, you can use part BBa_K788010 and BBa_K7880005 to make new binary vectors. And you may put the signature on the plant you work with by adding a barcode 2 part at the end of your system according to rules BBa_K880007 gave.

Retrieved from "http://2012.igem.org/Team:Nanjing-China"