Team:Exeter/lab book/3gip/wk6

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Latest revision as of 00:06, 27 September 2012

ExiGEM2012 Lab Book 3GP wk6


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 27th - 31st August - 3rd - 7th September - 10th - 14th September - 17th - 21st September - Results
	The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Tuesday 14th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Wednesday 15th August Morning •Miniprepping BBa_J13002 + wbnK BBa_B0014 in pSB1T3 wfcA + BBa_B0014 in pSB1T3 wbnJ + BBa_B0014 in pSB1T3 BBa_B0034 wbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August – Friday 17th August Preparation for presentation so no lab work.

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+      <a href="https://2012.igem.org/Team:Exeter/lab_book/proto"; style="color:#57b947">Protocols</a>
+       &nbsp;|&nbsp;
        <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
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          <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk11"; style="color:#1d1d1b">17th - 21st September</a>
+         <p>
+         -
+         </p>
+        <a href="https://2012.igem.org/Team:Exeter/Results/inducible"; style="color:#e30614" target="_blank"><font size="3"><b>Results</b></font></a>
+</font>
        </font>
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     <!------------INSERT WEEKLY IMAGE HERE------------>
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+      <font face="Verdana" color="#1d1d1b" size="2">
-       <font face="DokChampa" color="#57b947" size="4">
+       <font face="Verdana" color="#57b947" size="4">
         <p><b><u>The 3-Gene Inducible Plasmid: 13th - 17th August 2012</u></b></p>
        </font>
-      <!------------ENTER LAB BOOK HERE: PLEASE INCLUDE DATES WITHIN THE WEEK------------>
+      <p><b><u>Monday 13th August</u></b></p>
+<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
+<p></p><br>
+<p><b><u>Tuesday 14th August</u></b></p>
+<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
+<p></p><br>
+<p><b><u>Wednesday 15th August</u></b></p>
+<p><i><b>Morning</b></i></p>
+<p>•<a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57B947" target="_blank"><u>Miniprepping</u></a></p>
+<p>BBa_J13002 + <i>wbnK</i> BBa_B0014 in pSB1T3</p>
+<p><i>wfcA</i> + BBa_B0014 in pSB1T3</p>
+<p><i>wbnJ</i> + BBa_B0014 in pSB1T3</p>
+<p>BBa_B0034 <i>wbbC</i> + BBa_B0014 in pSB1T3</p>
+<p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p>
+<p>After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p>
+<p>40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. </p>
+<p>All were nanodropped and only <i>wfcA</i> + BBa_B0014 and <i>wbnJ</i> + BBa_B0014 gave good concentrations. </p><br>
+<p><b><u>Wednesday 15th August – Friday 17th August</u></b></p>
+<p>Preparation for presentation so no lab work. </p>
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+   <font color="#57B947" size="1" face="Verdana">
+    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
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