Orthogonal System

Based on the orthogonal ribosome and RBS calculator, we built our own orthogonal system. In the system, we use RFP and GFP to verify the feasibility. What is more, we build up a model to predict the output of GFP and RFP. It turned out the system works fine, and the result is constant with the prediction of our model.

Genetic Pollution Prevention and Genetic Encryption

Aiming at gene pollution, we employed the orthogonal system – O-Key – to prevent gene pollution. O-Key showed a promising application information encryption. Furthermore, the O-Key are applied to the entire organism to construct the bacteriophage. In addition, a successful interdisciplinary model was constructed to predict the diffusion of exogenous gene across space and time.

Logic Metabolism Regulation

We describe the principles of Yeast Assembler, and specifically used this method to construct the gene needed to produce Violacein. Through such a experiment, we could also prove the feasibilities of orthogonal system in regulating metabolism. Furthermore, we talked about the application of And gate based on orthogonal system in adjusting metabolism.