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<!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | <!-- https://2012.igem.org/Team:EPF-Lausanne/Protocol --> | ||
| - | We use the QIAGEN QIAprep Spin Miniprep Kit with their [http://www.qiagen.com/literature/render.aspx?id=370 protocol] and a microcentrifuge. | + | Pellets resuspended with RNase containing buffer (Resuspension Buffer R3, equivalent to Buffer P1, in Swomya's box in the fridge). |
| + | Note: keep the buffer in ice if you are not bringing it back to the fridge for some minutes. | ||
| + | |||
| + | We then use the QIAGEN QIAprep Spin Miniprep Kit with their [http://www.qiagen.com/literature/render.aspx?id=370 protocol] (page 22) and a microcentrifuge. | ||
{{:Team:EPF-Lausanne/Template/ProtocolFooter}} | {{:Team:EPF-Lausanne/Template/ProtocolFooter}} | ||
<noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | <noinclude>{{:Team:EPF-Lausanne/Template/Footer}}</noinclude> | ||
Pellets resuspended with RNase containing buffer (Resuspension Buffer R3, equivalent to Buffer P1, in Swomya's box in the fridge).
Note: keep the buffer in ice if you are not bringing it back to the fridge for some minutes.
We then use the QIAGEN QIAprep Spin Miniprep Kit with their [http://www.qiagen.com/literature/render.aspx?id=370 protocol] (page 22) and a microcentrifuge.
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