Team:ULB-Brussels/Conclusion

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<center><font color="#000000"; size="100"> Team ULB-Brussels, conclusion &  </font></center>
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<br></br><center><font color="#000000"; size="100"> perspectives!  </font></center>
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<p><h1>Construction of the integron</h1>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As said in the introduction, an integron is composed of different gene cassettes located downstream of a recombinase-encoding conserved sequence. To allow genes rearrangement, they must be flanked by attc sites. Indeed, these sites are recognized by the recombinase that allows the excision of the different gene cassettes and their integration in another <em>attc</em> site. In the results, we showed that we had to add an RFC10 prefix and a RBS sequence upstream of each gene and an <em>attc</em> site followed by a RFC10 suffix downstream of the gene. In the future, we should build the remaining microcine biobricks. The next step will be to assemble all the biobrick parts of the Microcin B17 and C7. Those genes could be assembled in the natural or in a random order. Thereafter, the integron biobrick as to be put in an iGEM plasmid provided by Mazel D. named . This plasmid contains –between the promoter and the RFC10 region – an <em>attI</em> site which required for the recombination by the integrase to occur.
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<p><h1>Competitions</h1>
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Revision as of 22:34, 26 September 2012

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Team ULB-Brussels, conclusion &


perspectives!


Construction of the integron

     As said in the introduction, an integron is composed of different gene cassettes located downstream of a recombinase-encoding conserved sequence. To allow genes rearrangement, they must be flanked by attc sites. Indeed, these sites are recognized by the recombinase that allows the excision of the different gene cassettes and their integration in another attc site. In the results, we showed that we had to add an RFC10 prefix and a RBS sequence upstream of each gene and an attc site followed by a RFC10 suffix downstream of the gene. In the future, we should build the remaining microcine biobricks. The next step will be to assemble all the biobrick parts of the Microcin B17 and C7. Those genes could be assembled in the natural or in a random order. Thereafter, the integron biobrick as to be put in an iGEM plasmid provided by Mazel D. named . This plasmid contains –between the promoter and the RFC10 region – an attI site which required for the recombination by the integrase to occur.

Competitions







    




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