Team:Dundee/Modelling2

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<li class='has-sub'><a href='https://2012.igem.org/Team:Dundee/Wet Lab'><span>Wet Lab</span></a>
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<h3>How effective is endolysin at killing <i>C. difficile</i>?</h3><br>
 
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Different Endolysin concentrations can have different effects on the survival of <i>C.difficile</i> cells. It is important therefore to understand how much endolysin is required to control <i>C.difficile</i> population and how can this concentration be obtained from our newly engineered <i>E.coli</i>. In this section we present mathematical models and quantative analyses that quantify the effect of Endolysin on a <i>C.difficile</i> population, Models were constructed using data that have been collected over summer, but we have also exploited data from the literature.<br><br>
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First, we used data from “<i>Molecular Characterization of a Clostridium difficile Bacteriophage and Its Cloned Biologically Active Endolysin</i>” where the size of the <i>C.difficile</i> population is measured (optical density, OD) as a function of the Endolysin concentration, E(μg/ml) and the time, t(mins) during which <i>C.difficile</i> is exposed to Endolysin.<br><br>
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Latest revision as of 22:26, 26 September 2012