Team:Dundee/Modelling2

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<li class='has-sub'><a href='https://2012.igem.org/Team:Dundee/Wet Lab'><span>Wet Lab</span></a>
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<h3>How effective is endolysin at killing <i>C. difficile</i>?</h3><br>
 
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Different Endolysin concentrations can have different effects on the survival of C.diff cells. It is important therefore to understand how much endolysin is required to control C.diff population and how can this concentration be obtained from our newly engineered E.coli. In this section we present mathematical models and quantative analyses that quantify the effect of Endolysin on a C.diff population, Models were constructed using data that have been collected over summer, but we have also exploited data from the literature.<br><br>
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First, we used data from“Molecular Characterization of a Clostridium difficile Bacteriophage and Its Cloned Biologically Active Endolysin” where the size of the C.diff population is measured (optical density, OD) as a function of the Endolysin concentration, E(μg/ml) and the time, t(mins) during which C.diff is exposed to Endolysin.<br><br>
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Latest revision as of 22:26, 26 September 2012