Team:LMU-Munich/Results

From 2012.igem.org

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===Finished and evaluated 5 ''B. subtilis'' vectors in BioBrick standard!===
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===Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.===
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===Sporobeads - what protein do you want them to display?===
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===Sporobeads - what protein do you want to display?===
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<font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. W168 showed near 100% germination rates. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Our results were consistent with this expectation. Mutants used were: <br />
<font color="#000000"; size="2">'''The germination rates of ''B. subtilis'' germination mutants.''' All germination rates are relative to wild type 168 (W168), our positive control. W168 showed near 100% germination rates. The mutant ''spo0A''::tet is unable to form spores, and should therefore show no germination (our negative control). Our results were consistent with this expectation. Mutants used were: <br />
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'''W168''': Wild-type 168 <br />
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'''W168''': wild-type 168 <br />
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'''''spo0A''::tet''': A sporulation-deficient strain <br />
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'''''spo0A''::tet''': a sporulation-deficient strain <br />
'''B29''': ''cwlD''::kan, ''sleB''::mls <br />
'''B29''': ''cwlD''::kan, ''sleB''::mls <br />
'''B30''': ''gerD''::cm, ''sleB''::mls <br />
'''B30''': ''gerD''::cm, ''sleB''::mls <br />
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'''B43''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec <br />
'''B43''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec <br />
'''B46''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm, ''sleB''::mls <br />
'''B46''': ''cwlD''::kan, ''cwlJ''::spec, ''gerD''::cm, ''sleB''::mls <br />
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'''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan<br>
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'''B47''': ''gerD''::cm, ''sleB''::mls, ''cwlJ''::spec, ''cwlD''::kan<br></font>
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The remaining germination ability in strain B30 seems inconsistent with the retained germination ability in strain B42 because B42 contains mutations to only one of the genes knocked out in B30. For example, we would have expected that B41 or B43, which contain both ''gerD''::cm and ''sleB''::mls should retain some germination ability instead. We could find no evidence of a labeling mix-up that could have caused the inconsistency. The germination of B42 spores occurred in only one of the many germination assays performed on this strain.</font>
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<p align="justify">*No germinating spores detected in 4.6x10<sup>9</sup> spores.</p>
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<p align="justify">*No germinating spores detected in 4.6x10<sup>9</sup> spores. For a full explanation of these great results, see [https://2012.igem.org/Team:LMU-Munich/Data/Knockout All results].</p>

Latest revision as of 22:13, 26 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU red and white.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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