Team:KAIT Japan/Notebook

From 2012.igem.org

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__NOTOC__
__NOTOC__
='''Notebook'''=
='''Notebook'''=
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:Our team pushed forward an experiment while making trial and error every day.
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:We made an experiment every day. It was process of trial and error.
-
=='''Creating parts of Tar methylation region'''==
+
=='''Creating parts of Methylation and Azurin'''==
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:<big>'''Date:8/9'''</big>
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:<big>'''Date:8/9'''</big> PCR
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::Colony PCR
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:::→Reflection:We Took ''E.coli'' too many.You should take less ''E.coli''.<br>
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-
:::→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)
+
-
:<big>'''Date:8/11'''</big>
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:<big>'''Date:8/10'''</big> Electrophoresis
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::The purified DNA
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-
:::→Reflection:Band was less.
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::PCR Product
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:<big>'''Date:8/13'''</big>
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:<big>'''Date:8/13'''</big> Electrophoresis and The refinement of DNA,PCR
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::Confirmed of electrophoresis by PCR product and Ligation of the TA vector
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:<big>'''Date:8/14'''</big>
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:<big>'''Date:8/14'''</big> Transformation
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::Transformation
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-
:<big>'''Date:8/25'''</big>
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:<big>'''Date:8/16'''</big> Blue white selection and Miniprep,Colony PCR
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::Miniprep
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-
:<big>'''Date:9/10'''</big>
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:<big>'''Date:8/17'''</big> Electrophoresis and Colony PCR
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::
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-
:<big>'''Date:9/11'''</big>
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:<big>'''Date:8/18'''</big> PCR and Electrophoresis
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::Primer design
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-
:<big>'''Date:9/12'''</big>
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:<big>'''Date:8/20'''</big> Extraction and the refinement of DNA
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::Miniprep,Ligation
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:<big>'''Date:9/13'''</big>
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:<big>'''Date:8/21'''</big> PCR and Electrophoresis,Transformation
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::Electrophoresis,Refinement of DNA
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:<big>'''Date:9/14'''</big>
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:<big>'''Date:8/22'''</big> Electrophoresis
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::Colony PCR
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:<big>'''Date:9/15'''</big>
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:<big>'''Date:8/23'''</big> PCR
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::Ligation
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:<big>'''Date:9/16'''</big>
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:<big>'''Date:8/25'''</big> Miniprep and Restriction enzyme processing
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::PCR,Ligation
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:<big>'''Date:9/17'''</big>
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:<big>'''Date:8/27'''</big> Electrophoresis
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::PCR,Ligation
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:<big>'''Date:9/19'''</big>
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:<big>'''Date:8/28'''</big> Electrophoresis and The refinement of DNA,PCR
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::Ligation
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:<big>'''Date:9/20'''</big>
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:<big>'''Date:8/29'''</big> Electrophoresis
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::Enzyme inactivation,Ligation
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-
:<big>'''Date:9/22'''</big>
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:<big>'''Date:8/30'''</big> Electrophoresis and The refinement of DNA
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::Transformation,Colony PCR,Electrophoresis,Miniprep
+
-
:<big>'''Date:9/23'''</big>
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:<big>'''Date:9/1'''</big> Extraction of DNA
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::Colony PCR,Electrophoresis,Streak
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-
:<big>'''Date:9/25'''</big>
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:<big>'''Date:9/2'''</big> Redesign of the primer
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::Colony PCR
+
-
=='''Creating parts of azurin'''==
+
:<big>'''Date:9/3'''</big> PCR and Restriction enzyme processing,Electrophoresis
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:<big>'''Date:8/16'''</big>
+
-
::Colony PCR
+
-
::Ligation
+
-
:<big>'''Date:8/18'''</big>
+
:<big>'''Date:9/5'''</big> Miniprep
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::Colony PCR
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-
:<big>'''Date:8/20'''</big>
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:<big>'''Date:9/6'''</big> Electrophoresis
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::DNA extraction and purification of ''P.aeruginosa''
+
 
 +
:<big>'''Date:9/10'''</big> Colony PCR
 +
 
 +
:<big>'''Date:9/12'''</big> Miniprep
 +
 
 +
:<big>'''Date:9/14'''</big> PCR
 +
 
 +
:<big>'''Date:9/15'''</big> Restriction enzyme processing
 +
 
 +
:<big>'''Date:9/16'''</big> PCR
 +
 
 +
:<big>'''Date:9/17'''</big> PCR
 +
 
 +
:<big>'''Date:9/19'''</big> PCR and Restriction enzyme processing
 +
 
 +
:<big>'''Date:9/20'''</big> Ligation
 +
 
 +
:<big>'''Date:9/22'''</big> Transformation
 +
 
 +
:<big>'''Date:9/23'''</big> PCR and Electrophoresis
 +
 
 +
:<big>'''Date:9/24'''</big> Ligation
 +
 
 +
:<big>'''Date:9/25'''</big> Transformation
 +
 
 +
:<big>'''Date:9/27'''</big> Miniprep and Electrophoresis
 +
 
 +
=='''Creating parts of Tar'''==
 +
:<big>'''Date:9/13'''</big> PCR and Colony PCR
 +
 
 +
:<big>'''Date:9/14'''</big> Electrophoretic confirmation
 +
 
 +
:<big>'''Date:9/15'''</big> Restriction enzyme processing
 +
 
 +
:<big>'''Date:9/16'''</big> Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
 +
 
 +
:<big>'''Date:9/17'''</big> Refinement of DNA and Ligation,Transformation
 +
 
 +
:<big>'''Date:9/18'''</big> Making of a master plate and Preculture,Recultivation
 +
 
 +
:<big>'''Date:9/19'''</big> Colony PCR and Electrophoresis
 +
 
 +
:<big>'''Date:9/20'''</big> Ligation and Transformation
 +
 
 +
:<big>'''Date:9/21'''</big> Inoculation
 +
 
 +
:<big>'''Date:9/22'''</big> Making of a master plate and Colony PCR,Electrophoresis,Preculture
 +
 
 +
:<big>'''Date:9/23'''</big> Electrophoretic confirmation and Electrophoresis of the PCR
 +
 
 +
:<big>'''Date:9/24'''</big> Miniprep and Electrophoresis
 +
 
 +
:<big>'''Date:9/25'''</big> PCR of the miniprep product and Electrophoresis
 +
 
 +
=='''Creating parts of EnvZ'''==
 +
:<big>'''Date:9/10'''</big> Design of the primer and PCR,Elecrtophoresis,Miniprep
 +
 
 +
:<big>'''Date:9/13'''</big> PCR and Elecrtophoresis
 +
 
 +
:<big>'''Date:9/18'''</big> Design of the primer
 +
 
 +
:<big>'''Date:9/19'''</big> PCR and Elecrtophoresis
 +
 
 +
:<big>'''Date:9/20'''</big> Restriction enzyme processing
 +
 
 +
:<big>'''Date:9/21'''</big> Ligation and PCR,Elecrtophoresis
 +
 
 +
:<big>'''Date:9/23'''</big> PCR
 +
 
 +
:<big>'''Date:9/24'''</big> The refinement of DNA and Restriction enzyme processing,Ligation
 +
 
 +
:<big>'''Date:9/25'''</big> Transformation
 +
 
 +
:<big>'''Date:9/26'''</big> Liquid culture
|}
|}

Latest revision as of 22:12, 26 September 2012

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Home

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice

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Team

Notebook

We made an experiment every day. It was process of trial and error.

Creating parts of Methylation and Azurin

Date:8/9 PCR
Date:8/10 Electrophoresis
Date:8/13 Electrophoresis and The refinement of DNA,PCR
Date:8/14 Transformation
Date:8/16 Blue white selection and Miniprep,Colony PCR
Date:8/17 Electrophoresis and Colony PCR
Date:8/18 PCR and Electrophoresis
Date:8/20 Extraction and the refinement of DNA
Date:8/21 PCR and Electrophoresis,Transformation
Date:8/22 Electrophoresis
Date:8/23 PCR
Date:8/25 Miniprep and Restriction enzyme processing
Date:8/27 Electrophoresis
Date:8/28 Electrophoresis and The refinement of DNA,PCR
Date:8/29 Electrophoresis
Date:8/30 Electrophoresis and The refinement of DNA
Date:9/1 Extraction of DNA
Date:9/2 Redesign of the primer
Date:9/3 PCR and Restriction enzyme processing,Electrophoresis
Date:9/5 Miniprep
Date:9/6 Electrophoresis
Date:9/10 Colony PCR
Date:9/12 Miniprep
Date:9/14 PCR
Date:9/15 Restriction enzyme processing
Date:9/16 PCR
Date:9/17 PCR
Date:9/19 PCR and Restriction enzyme processing
Date:9/20 Ligation
Date:9/22 Transformation
Date:9/23 PCR and Electrophoresis
Date:9/24 Ligation
Date:9/25 Transformation
Date:9/27 Miniprep and Electrophoresis

Creating parts of Tar

Date:9/13 PCR and Colony PCR
Date:9/14 Electrophoretic confirmation
Date:9/15 Restriction enzyme processing
Date:9/16 Electrophoresis of the restriction enzyme processing product and Restriction enzyme processing
Date:9/17 Refinement of DNA and Ligation,Transformation
Date:9/18 Making of a master plate and Preculture,Recultivation
Date:9/19 Colony PCR and Electrophoresis
Date:9/20 Ligation and Transformation
Date:9/21 Inoculation
Date:9/22 Making of a master plate and Colony PCR,Electrophoresis,Preculture
Date:9/23 Electrophoretic confirmation and Electrophoresis of the PCR
Date:9/24 Miniprep and Electrophoresis
Date:9/25 PCR of the miniprep product and Electrophoresis

Creating parts of EnvZ

Date:9/10 Design of the primer and PCR,Elecrtophoresis,Miniprep
Date:9/13 PCR and Elecrtophoresis
Date:9/18 Design of the primer
Date:9/19 PCR and Elecrtophoresis
Date:9/20 Restriction enzyme processing
Date:9/21 Ligation and PCR,Elecrtophoresis
Date:9/23 PCR
Date:9/24 The refinement of DNA and Restriction enzyme processing,Ligation
Date:9/25 Transformation
Date:9/26 Liquid culture
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