"
Team:SJTU-BioX-Shanghai
From 2012.igem.org
Huanan1991 (Talk | contribs) |
Huanan1991 (Talk | contribs) |
||
| Line 655: | Line 655: | ||
<p>We aimed at constructing a set of protein scaffolds on E.coli cell membrane as carriers of various enzymes. Distinct from previous DNA or RNA scaffold construction, our device limits the reaction space to a two-dimensional surface. In such system,the membrane replaced DNA or RNA scaffold as an extensive surface/scaffold for proteins to anchor without limitation of scaffold amount. </p> | <p>We aimed at constructing a set of protein scaffolds on E.coli cell membrane as carriers of various enzymes. Distinct from previous DNA or RNA scaffold construction, our device limits the reaction space to a two-dimensional surface. In such system,the membrane replaced DNA or RNA scaffold as an extensive surface/scaffold for proteins to anchor without limitation of scaffold amount. </p> | ||
| - | <p>By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of Fatty Acid was enhanced by more than 20 | + | <p>By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of Fatty Acid was enhanced by more than 20 fold through recruiting our membrane accelerator system, which has a promising application prospect in biofuel production.</p> |
<p>More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device.</p> | <p>More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device.</p> | ||
Revision as of 21:52, 26 September 2012
|
Abstract: This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled. We aimed at constructing a set of protein scaffolds on E.coli cell membrane as carriers of various enzymes. Distinct from previous DNA or RNA scaffold construction, our device limits the reaction space to a two-dimensional surface. In such system,the membrane replaced DNA or RNA scaffold as an extensive surface/scaffold for proteins to anchor without limitation of scaffold amount. By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of Fatty Acid was enhanced by more than 20 fold through recruiting our membrane accelerator system, which has a promising application prospect in biofuel production. More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device. |
|
