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Team:Exeter/lab book/3gip/wk6
From 2012.igem.org
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Revision as of 21:48, 26 September 2012
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The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Tuesday 14th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Wednesday 15th August Morning BBa_J13002 + wbnK BBa_B0014 in pSB1T3 wfcA + BBa_B0014 in pSB1T3 wbnJ + BBa_B0014 in pSB1T3 BBa_B0034 wbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August – Friday 17th August Preparation for presentation so no lab work. |
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