Team:LMU-Munich/Data

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Revision as of 21:45, 26 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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Data

Here you will find all of our project data.

BacillusBioBrickBox

Evaluation of B. subtilis vectors


Seven novel vectors were constructed and four already proven to work in B. subtilis.

Anderson Promoter Evaluation


Eleven Anderson promoters were measured in B. subtilis and showed relatively weak activity.

Constitutive Bacillus Promoters


Three novel B. subtilis promoter BioBricks (P_liaG, P_veg, P_lepA) were constructed and functionality verified by luminescence and β-galactosidase assays.

Inducible Bacillus Promoters


A novel antibiotic-inducible promoter, P_liaI, was constructed and thoroughly evaluated.

Sporobeads

Crust Promoter Evaluation


Three sporulation-dependent promoters (P_cotyz, P_cotv, P_cgeA) were constructed, two of which show the expected behavior.

GFP-Sporobead Evaluation


They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.

Sporobead Purification


We compared different ways of purifying Bacillus spores from vegetative cells: Lysozyme is the best!

GerminationSTOP

Knockouts of germination genes


We successfully prevented spore germination by multiple gene knockouts.

Suicide Switch


Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.

Inverter

β-galactosidase assay of Inverter with lacZα


It works! Invert the output of your promoter of choice.

Trashcan

Things that were a pain in the neck!

cgeA and P_cgeA


Unexpected restriction sites combined with no activity at all - what a bummer!

MazF

The story of trying to clone a toxine from E. coli in E. coli: They die, dammit! (...not a surprise, really...)

The story of trying to clone a toxine from E. coli in E. coli: They die, dammit! (...not a surprise, really...)

Xylose promoters

All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).

All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).

For our big breakthroughs, or to follow specific projects, see the individual project pages:

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Data"

@@ Line 36: / Line 36: @@
 ====Constitutive ''Bacillus'' Promoters====
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks ('''P<sub>''liaG''</sub>''', '''P<sub>''veg''</sub>''', '''P<sub>''lepA''</sub>''') were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
+|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
 |[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
 |-
@@ Line 45: / Line 45: @@
 ====Inducible ''Bacillus'' Promoters====
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">A novel antibiotic-inducible promoter, '''P<sub>''liaI''</sub>''', was constructed and thoroughly evaluated.</p>
+|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
 |[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
 |-
@@ Line 61: / Line 61: @@
 ===Crust Promoter Evaluation===
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Three sporulation-dependent promoters ('''P<sub>''cotyz''</sub>''', '''P<sub>''cotv''</sub>''', '''P<sub>''cgeA''</sub>''') were constructed, two of which show the expected behavior.</p>
+|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotyz''</sub>, P<sub>''cotv''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
 |[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
 |-