Team:Exeter/Achievements

• Successfully cloned and submitted 14 biobricks into the registry
• Successfully cloned over 32 more Biobricks waiting to be transferred into pSB1C3 plasmids
• Using PCR we obtained wbbC gene from BL21 genome
• Created GlycoBase; a database containing a list of over 100 different glycosyltransferase enzymes mainly from E.coli strains demonstrating the vast linkages we could achieve with just E.coli enzymes
• Created GlycoWeb, an interface for an online user to access Glycobase. Glycoweb has the ability to tell you which enzymes are needed to create your bespoke polysaccharide.
• Created GlycoApp; an application for a handheld device to access the Glycobase giving the user distance access and enhanced ordering capabilities
• The project evolved with human practices for the consideration of the ethical, societal, environmental and business aspects of our project. We held a human practice panel, Café Scientifique talk, met with various company members from different business sectors, led an A level master class and worked with several work experience students.
• The discussions with various businesses had a very positive response and we received several letters of support.
• We created devices to test our proteins, test our natural polysaccharides and to help construct our 3 gene inducible plasmid. These included BBa_K094120_BBa_B0034_wclY_BBaB0014, BBa_J13002_wbnk_BBaB0014, BBa_J23119_BBa_B0034_ wbnJ_BBaB0014 BBa_J13002_ompA_BBa_J322921_BBa_B0034 and BBa_K20600_ompA_BBa_J322921_BBa_B0034.
• We managed to obtain 7 of 9 fragments for Gibson assembly through PCR. The different annealing temperatures of the primers made this hard but showed how important the preparation for Gibson has to be

Nearly There!!

• In touching distance of completing our operons. Only time stopped this happening.
• We were one construct away from being able to complete our 3 gene inducible plasmid.
• We were not able to fully test our constructs and properly characterise our biobricks.