Recipes

• Preparation of LB Medium
• Competent Cell Preparation
• TAE Production
• Agar Plates Preparation

Cloning

• PCR
• PCR Cleanup
• Gel Electrophoresis
• Gel Extraction
• Restriction Site Digestion
• Quick and Dirty Digestion
• Ligation
• E.Coli Transformation
• Colony PCR
• Prepare Plasmid Extraction
• Miniprep
• Maxiprep

Mammalian

• Cell Passaging
• CHO Transfection
• HEK Transfection
• Trypan Blue Method

Expression

• Western Blot
• Flow Cytometry (Guava)
• Sampling for Tecan machine
• Silver Staining
• Calcium imaging
• Microscopy
• Flow Cytometry
• mRNA Extraction
• RT-PCR

DNA Quality

• DNA Concentration Measurement
• DNA Sequencing Sample Preparation