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'''Cloning''' | '''Cloning''' | ||
* [[Team:EPF-Lausanne/Protocol/PCR|PCR]] | * [[Team:EPF-Lausanne/Protocol/PCR|PCR]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/PCRCleanup|PCR Cleanup]] | ||
* [[Team:EPF-Lausanne/Protocol/Gel|Gel Electrophoresis]] | * [[Team:EPF-Lausanne/Protocol/Gel|Gel Electrophoresis]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/GelExtraction|Gel Extraction]] | ||
* [[Team:EPF-Lausanne/Protocol/RestrictionSiteDigestion|Restriction Site Digestion]] | * [[Team:EPF-Lausanne/Protocol/RestrictionSiteDigestion|Restriction Site Digestion]] | ||
* [[Team:EPF-Lausanne/Protocol/QuickAndDirtyRestrictionDigestion|Quick and Dirty Digestion]] | * [[Team:EPF-Lausanne/Protocol/QuickAndDirtyRestrictionDigestion|Quick and Dirty Digestion]] | ||
* [[Team:EPF-Lausanne/Protocol/Ligation|Ligation]] | * [[Team:EPF-Lausanne/Protocol/Ligation|Ligation]] | ||
| - | * [[Team:EPF-Lausanne/Protocol/Transformation|E. | + | * [[Team:EPF-Lausanne/Protocol/Transformation|E.Coli Transformation]] |
| + | * [[Team:EPF-Lausanne/Protocol/ColonyPCR|Colony PCR]] | ||
* [[Team:EPF-Lausanne/Protocol/PreparePlasmidExtraction|Prepare Plasmid Extraction]] | * [[Team:EPF-Lausanne/Protocol/PreparePlasmidExtraction|Prepare Plasmid Extraction]] | ||
* [[Team:EPF-Lausanne/Protocol/MiniPrep|Miniprep]] | * [[Team:EPF-Lausanne/Protocol/MiniPrep|Miniprep]] | ||
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'''Mammalian''' | '''Mammalian''' | ||
| + | * [[Team:EPF-Lausanne/Protocol/Passaging|Cell Passaging]] | ||
* [[Team:EPF-Lausanne/Protocol/TransfectionCHO|CHO Transfection]] | * [[Team:EPF-Lausanne/Protocol/TransfectionCHO|CHO Transfection]] | ||
* [[Team:EPF-Lausanne/Protocol/TransfectionHEK|HEK Transfection]] | * [[Team:EPF-Lausanne/Protocol/TransfectionHEK|HEK Transfection]] | ||
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'''Expression''' | '''Expression''' | ||
* [[Team:EPF-Lausanne/Protocol/Western_Blot|Western Blot]] | * [[Team:EPF-Lausanne/Protocol/Western_Blot|Western Blot]] | ||
| - | * [[Team:EPF-Lausanne/Protocol/Flowcytometry-Guava| | + | * [[Team:EPF-Lausanne/Protocol/Flowcytometry-Guava|Flow Cytometry (Guava)]] |
* [[Team:EPF-Lausanne/Protocol/Sampling for Tecan machine|Sampling for Tecan machine]] | * [[Team:EPF-Lausanne/Protocol/Sampling for Tecan machine|Sampling for Tecan machine]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/Silver staining|Silver Staining]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/Calcium|Calcium imaging]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/Microscopy|Microscopy]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/FACS|Flow Cytometry]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/MRnaExtraction|mRNA Extraction]] | ||
| + | * [[Team:EPF-Lausanne/Protocol/rtPCR|RT-PCR]] | ||
Recipes
Cloning
Mammalian
Expression
DNA Quality
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