Team:XMU-China/protocolsindex

From 2012.igem.org

(Difference between revisions)

Latest revision as of 19:36, 26 September 2012

safetyindex

Contents[hide] [show]
1 General protocols
1.1 Stock solution
1.2 Preparation of Competent BL21
1.3 Transformation
1.4 Plasmid Purification
1.5 Reaction system of restriction endonuclease
1.6 Standard BioBrick Assembly
1.7 Suffix Insertion
1.8 Ligation
1.9 Restriction analysis
1.10 Gel Extraction
2 Characterization
2.1 Fluorescence Measurements
2.2 Protein electrophoresis
2.2.1 Preparation of Samples
2.2.2 Manufacture Albumen Gel
2.2.3 Electrophoresis
2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
2.2.5 Scanning
3 Immobilization
3.1 Prepare Sodiumcellu-losesulfate (NaCS)
3.2 Prepare microcapsules
3.3 Immobilize cells into calcium alginate beads
3.4 Immobilize cells into intra-hollow Ca-alginate capsules
3.5 Immobilize cells into NaCS-PDMDAAC microcapsules

@@ Line 18: / Line 18: @@
 <body>
-   <table width="900" border="3" id="contents">
+   <table width="900" border="3"  id="contents">
      <tr>
        <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span>
@@ Line 26: / Line 26: @@
      <tr>
-       <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01">1.&nbsp; <strong> General Protocols</strong></a></li> </td>
+       <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01"><strong>1 General protocols</strong></a></li> </td>
      </tr>
      <tr>
-       <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2.&nbsp;&nbsp;Characterization</strong></a></li> </td>
+       <td id="indextd2"><li id="indexli"><a id="indexa" href="#_Toc02"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.1&nbsp;Stock solution</strong></a></li></td>
      </tr>
      <tr>
-       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3.&nbsp;&nbsp; Immobilization</strong></a></li> </td>
+      <td id="indextd3"><li id="indexli"><a id="indexa" href="#_Toc03"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.2&nbsp;Preparation of Competent BL21</strong></a><a href="#_Toc03"> </a></li></td>
+    </tr>
+    <tr>
+      <td id="indextd4"><li id="indexli"><a id="indexa" href="#_Toc04"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.3&nbsp;Transformation</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd5"><li id="indexli"><a id="indexa" href="#_Toc05"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.4&nbsp;Plasmid Purification</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd6"><li id="indexli"><a id="indexa" href="#_Toc06"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.5&nbsp;Reaction system of restriction endonuclease</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd7"><li id="indexli"><a id="indexa" href="#_Toc07"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.6&nbsp;Standard  BioBrick Assembly</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd8"><li id="indexli"><a id="indexa" href="#_Toc08"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.7&nbsp;Suffix  Insertion</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd9"><li id="indexli"><a id="indexa" href="#_Toc09"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.8&nbsp;Ligation</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd10"><li id="indexli"><a id="indexa" href="#_Toc10"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.9&nbsp;Restriction  analysis</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd11"><li id="indexli"><a id="indexa" href="#_Toc11"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.10&nbsp;Gel  Extraction</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2 Characterization</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd13"><li id="indexli"><a id="indexa" href="#_Toc13"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.1&nbsp;Fluorescence Measurements </strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd14"><li id="indexli"><a id="indexa" href="#_Toc14"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2&nbsp;Protein electrophoresis</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd15"><li id="indexli"><a id="indexa" href="#_Toc15"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.1&nbsp;Preparation of Samples</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc16"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.2&nbsp;Manufacture Albumen Gel</strong></a></li> </td>
+    </tr>
+     <tr>
+      <td id="indextd17"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd18"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
+    </tr>
+      <td id="indextd19"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
+    </tr>
+    <tr>
+       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd21"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd22"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd23"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
+    </tr>
+    <tr>
+      <td id="indextd24"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
+    </tr>
+      <tr>
+      <td id="indextd25"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
      </tr>