Team:Trieste/parts

From 2012.igem.org

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<h2>Primers </h2>
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<table>
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<thead>
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<tr>
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<th>Name</th>
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<th>Seq 5'--> 3'</th>
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<th>Notes</th>
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</tr>
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</thead>
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<tr><td>Vr</td>              <td>ATTACCGCCTTTGAGTGAGC</td>      <td>95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr>
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<tr><td>Vf2</td>              <td>TGCCACCTGACGTCTAAGAA</td>    <td> 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr>
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<tr><td>GlmSTn7Fw</td>        <td>GCATGTGGAAGAGGTGATTG</td>      <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>GlmSTn7Rev</td>      <td>ACTTTATTGTTCGCCCACA</td>            <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>B0015XhoRev</td>      <td>ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC</td>    <td>95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>B0015EcoFw</td>      <td>CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG</td>  <td>95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>T5EcoFw</td>          <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td>  <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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<tr><td>T5XhoRev</td>        <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td>  <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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</table>
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    <!--  ******************** CONTENUTO QUI ****************** -->  
    <!--  ******************** CONTENUTO QUI ****************** -->  
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Revision as of 19:18, 26 September 2012

Parts Overview

More

List of the submitted Parts

Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.



Name Type Description
BBa_K875001 Regulatory T5 Cumate Operator
BBa_K875002 Regulatory T5 Lac Operator
BBa_K875003 Composit CymR
BBa_K875004 Composit T5LacO-LPP-OmpA-scFv
BBa_K875005 Composit T5LacO-LPP-OmpA-SIP
BBa_K875006 Composit T5LacO-LPP-PelB-scFv
BBa_K875007 Composit T5 LacO-LPP-PelB-SIP
BBa_K875008 Coding IPTG inducible Tse2 toxin
BBa_K875009 Coding LL 37 - Cathelicidin
BBa_K875020 Composit β-Glucosidase

Primers

Name Seq 5'--> 3' Notes
Vr ATTACCGCCTTTGAGTGAGC 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞
Vf2 TGCCACCTGACGTCTAAGAA 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞
GlmSTn7Fw GCATGTGGAAGAGGTGATTG 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
GlmSTn7Rev ACTTTATTGTTCGCCCACA 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
B0015XhoRev ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC 95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
B0015EcoFw CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG 95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
T5EcoFw CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
T5XhoRev TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
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