Team:Groningen/Notebook/Wetwork 22August2012

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Yonathan and Elbrich
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Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.
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Violacein had not the right size, so ligation of the construct was not possible.
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Yonathan and Elbrich<br>
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Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.<br>
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Violacein did not have the right size, so ligation of the construct was not possible.<br>
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<br>
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Emeraldo<br>
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Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator (only psac from colony 3) with ligation reaction, E. coli DH5alpha transformation with the ligated product.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 18:42, 26 September 2012







Yonathan and Elbrich

Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.
Violacein did not have the right size, so ligation of the construct was not possible.

Emeraldo
Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator (only psac from colony 3) with ligation reaction, E. coli DH5alpha transformation with the ligated product.

Back to notebook

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