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Team:Groningen/Notebook/Wetwork 22August2012
From 2012.igem.org
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Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.<br> | Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.<br> | ||
| - | Violacein | + | Violacein did not have the right size, so ligation of the construct was not possible.<br> |
<br> | <br> | ||
Emeraldo<br> | Emeraldo<br> | ||
Latest revision as of 18:42, 26 September 2012
Yonathan and Elbrich
Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.
Violacein did not have the right size, so ligation of the construct was not possible.
Emeraldo
Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator (only psac from colony 3) with ligation reaction, E. coli DH5alpha transformation with the ligated product.
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