Team:KAIT Japan/Results

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<P>[[File:KAIT_Japan2012_logo.png|left|link=Team:KAIT_Japan]][[File:KAIT_Japan2012_Sub.png|right|link=http://www.kait.jp/index2.php]][[File:Kaitjapan_iGEM_official.logo.png‎|right|link=http://ung.igem.org/Main_Page]]
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!align="center"|[[File:Kaitjapan_results.png|link=Team:KAIT_Japan/Results]]
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='''Results'''=
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=='''Cytoplasmic Region of Tar'''==
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:'''We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.'''
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:[[file:KAIT_Japan-Results-Tar1.jpg]]
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:'''Figure1.PCR production from BBa_J22010'''
 +
:'''enzyme tag EcoRⅠand PstⅠ　We experimented as following.'''
 +
:'''1.restriction enzyme processing PCR production'''
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:'''2.ligation'''
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:'''3.transformation'''
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:'''4.culture on the LB nutrient medium and get colonies.'''
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:'''5.colony PCR and numbering colonies.'''
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:'''6.We did miniprep 1,2,4,5.this is colony number'''
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:
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:[[file:KAIT_Japan-Results-Tar2.jpg]] 
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:'''Figure2.the colony having　cytoplasmic region of tar '''
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:
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:'''7.After miniprep,we did PCR by using VF2 and VR primer'''
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:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
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:'''cytoplasmic region of tar bp is varied. 900bp~1000bp'''
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:
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:[[file:KAIT_Japan-Results-Tar3.jpg]] 
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:'''Figure3.PCR production by using VF and VR'''
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=='''Methylation Site of Tar'''==
 +
:
 +
:'''We did PCR so as to get methylation site of tar.We used primer with restriction '''
 +
:[[file:KAIT_Japan-Results-Met1.jpg‎]]
 +
:'''Figure1.PCR production from BBa_J22010'''
 +
 +
:'''enzyme tag EcoRⅠand PstⅠ　We experimented as following. '''
 +
:'''1.restriction enzyme processing PCR production '''
 +
:'''2.ligation '''
 +
:'''3.transformation'''
 +
:'''4.culture on the LB nutrient medium and get colonies.'''
 +
:'''5.colony PCR and numbering colonies.'''
 +
:'''6.We did miniprep 1,8,9,10.this is colony number'''
 +
:[[file:KAIT_Japan-Results-Met2.jpg]]
 +
:'''Figure2. the colony having methylation site of tar'''
 +
:
 +
:'''7.After miniprep,we did PCR by using VF2 and VR primer '''
 +
:'''8.The part of red boxes production can be seen the band of methylation site of tar. So,These miniprep production are tar parts.'''
 +
:'''methylation site of tar is 690bp'''
 +
:[[File:KAIT_Japan-Results-Met4.jpg]]
 +
:'''Figure3.PCR production by using VF and VR'''

Latest revision as of 18:15, 26 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Results

Cytoplasmic Region of Tar

We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.
KAIT Japan-Results-Tar1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ　We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,2,4,5.this is colony number
KAIT Japan-Results-Tar2.jpg
Figure2.the colony having　cytoplasmic region of tar
7.After miniprep,we did PCR by using VF2 and VR primer
8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.
cytoplasmic region of tar bp is varied. 900bp~1000bp
KAIT Japan-Results-Tar3.jpg
Figure3.PCR production by using VF and VR

Methylation Site of Tar

We did PCR so as to get methylation site of tar.We used primer with restriction
KAIT Japan-Results-Met1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ　We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,8,9,10.this is colony number
KAIT Japan-Results-Met2.jpg
Figure2. the colony having methylation site of tar
7.After miniprep,we did PCR by using VF2 and VR primer
8.The part of red boxes production can be seen the band of methylation site of tar. So,These miniprep production are tar parts.
methylation site of tar is 690bp
KAIT Japan-Results-Met4.jpg
Figure3.PCR production by using VF and VR
Retrieved from "http://2012.igem.org/Team:KAIT_Japan/Results"