Team:KAIT Japan/Results

From 2012.igem.org

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='''Results'''=
='''Results'''=
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=='''cytoplasmic region of tar'''==
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=='''Cytoplasmic Region of Tar'''==
:'''We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.'''
:'''We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.'''
:[[file:KAIT_Japan-Results-Tar1.jpg]]
:[[file:KAIT_Japan-Results-Tar1.jpg]]
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:'''Figure2.the colony having　cytoplasmic region of tar '''
:'''Figure2.the colony having　cytoplasmic region of tar '''
:
:
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:'''7.After miniprep we did PCR by using VF2 and VR primer'''  
+
:'''7.After miniprep,we did PCR by using VF2 and VR primer'''  
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
:'''cytoplasmic region of tar bp is varied. 900bp~1000bp'''
:'''cytoplasmic region of tar bp is varied. 900bp~1000bp'''
:
:
:[[file:KAIT_Japan-Results-Tar3.jpg]]   
:[[file:KAIT_Japan-Results-Tar3.jpg]]   
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:'''Figure3 PCR production by using VF and butVR'''
+
:'''Figure3.PCR production by using VF and VR'''
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=='''methylation site of tar'''==
+
=='''Methylation Site of Tar'''==
:
:
:'''We did PCR so as to get methylation site of tar.We used primer with restriction '''
:'''We did PCR so as to get methylation site of tar.We used primer with restriction '''
Line 86: Line 86:
:'''4.culture on the LB nutrient medium and get colonies.'''
:'''4.culture on the LB nutrient medium and get colonies.'''
:'''5.colony PCR and numbering colonies.'''
:'''5.colony PCR and numbering colonies.'''
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:'''6.We did miniprep 1,2,4,5.this is colony number'''
+
:'''6.We did miniprep 1,8,9,10.this is colony number'''
:[[file:KAIT_Japan-Results-Met2.jpg]]
:[[file:KAIT_Japan-Results-Met2.jpg]]
:'''Figure2. the colony having methylation site of tar'''  
:'''Figure2. the colony having methylation site of tar'''  
:
:
-
:'''7.After miniprep we did PCR by using VF2 and VR primer '''
+
:'''7.After miniprep,we did PCR by using VF2 and VR primer '''
-
:'''8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.'''
+
:'''8.The part of red boxes production can be seen the band of methylation site of tar. So,These miniprep production are tar parts.'''
:'''methylation site of tar is 690bp'''
:'''methylation site of tar is 690bp'''
-
:[[File:KAIT_Japan-Results-Met3.jpg]]
+
:[[File:KAIT_Japan-Results-Met4.jpg]]
-
:'''Figure3 PCR production by using VF and butVR'''
+
:'''Figure3.PCR production by using VF and VR'''

Latest revision as of 18:15, 26 September 2012

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Home

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice

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Team

Results

Cytoplasmic Region of Tar

We did PCR so as to get cytoplasmic region of tar.We used primer with restriction.
KAIT Japan-Results-Tar1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ　We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,2,4,5.this is colony number
KAIT Japan-Results-Tar2.jpg
Figure2.the colony having　cytoplasmic region of tar
7.After miniprep,we did PCR by using VF2 and VR primer
8.All production can be seen the band of cytoplasmic region of tar. So,These miniprep production are tar parts.
cytoplasmic region of tar bp is varied. 900bp~1000bp
KAIT Japan-Results-Tar3.jpg
Figure3.PCR production by using VF and VR

Methylation Site of Tar

We did PCR so as to get methylation site of tar.We used primer with restriction
KAIT Japan-Results-Met1.jpg
Figure1.PCR production from BBa_J22010
enzyme tag EcoRⅠand PstⅠ　We experimented as following.
1.restriction enzyme processing PCR production
2.ligation
3.transformation
4.culture on the LB nutrient medium and get colonies.
5.colony PCR and numbering colonies.
6.We did miniprep 1,8,9,10.this is colony number
KAIT Japan-Results-Met2.jpg
Figure2. the colony having methylation site of tar
7.After miniprep,we did PCR by using VF2 and VR primer
8.The part of red boxes production can be seen the band of methylation site of tar. So,These miniprep production are tar parts.
methylation site of tar is 690bp
KAIT Japan-Results-Met4.jpg
Figure3.PCR production by using VF and VR
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